Evidence map›Paper›PMID 40207931›Full record

ArticleJournal of virology2025

Cell binding, uptake, and infection of influenza A virus using recombinant antibody-based receptors.

Oluwafemi F Adu, Milagros Sempere Borau, Simon P Früh, Umut Karakus, Wendy S Weichert, Brian R Wasik, Silke Stertz, Colin R Parrish

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Oluwafemi F Adu *Department of Microbiology and Immunology, College of Veterinary Medicine, Baker Institute for Animal Health, Cornell University, Ithaca, New York, USA.ORCID 0000-0002-9914-6266
Milagros Sempere Borau *Institute of Medical Virology, University of Zurich, Zürich, Switzerland.ORCID 0000-0002-6614-5099
Simon P FrühDepartment of Microbiology and Immunology, College of Veterinary Medicine, Baker Institute for Animal Health, Cornell University, Ithaca, New York, USA.
Umut KarakusInstitute of Medical Virology, University of Zurich, Zürich, Switzerland.
Wendy S WeichertDepartment of Microbiology and Immunology, College of Veterinary Medicine, Baker Institute for Animal Health, Cornell University, Ithaca, New York, USA.
Brian R WasikDepartment of Microbiology and Immunology, College of Veterinary Medicine, Baker Institute for Animal Health, Cornell University, Ithaca, New York, USA.ORCID 0000-0001-5442-3883
Silke StertzInstitute of Medical Virology, University of Zurich, Zürich, Switzerland.ORCID 0000-0001-9491-2892
Colin R ParrishInstitute of Medical Virology, University of Zurich, Zürich, Switzerland.ORCID 0000-0002-1836-6655

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human and avian influenza A viruses bind to sialic acid (Sia) receptors on cells as their primary receptors, and this results in endocytic uptake of the virus. While the role of Sia on glycoproteins and/or glycolipids for virus entry is crucial, the roles of the carrier proteins are still not well understood. Furthermore, it is still unclear how receptor binding leads to infection, including whether the receptor plays a structural or other roles beyond being a simple tether. To enable the investigation of the receptor binding and cell entry processes in a more controlled manner, we have designed a protein receptor for pandemic H1 influenza A viruses. The engineered receptor possesses the binding domains of an anti-HA antibody prepared as a single-chain variable fragment (scFv) fused with the stalk, transmembrane, and cytoplasmic sequences of the feline transferrin receptor type-1 (fTfR). When expressed in cells that lack efficient display of Sia due to a knockout of the

Indexed as

Influenza A virusReceptors, VirusVirus InternalizationAnimalsAntibodies, ViralCatsCell LineDogsHEK293 CellsHemagglutinin Glycoproteins, Influenza VirusHumansInfluenza A Virus, H1N1 SubtypeInfluenza, HumanReceptors, TransferrinSingle-Chain AntibodiesVirus AttachmentAntibodies, ViralHemagglutinin Glycoproteins, Influenza VirusReceptors, TransferrinReceptors, VirusSingle-Chain Antibodiesendocytosisexperimental toolshost-cell interactionsorthomyxovirusreceptor binding

Identifiers

PMID40207931
PMCPMC12090727

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.