Evidence map›Paper›PMID 40205553›Full record

Observational studyBMC pulmonary medicine2025

Cannabis smoking is associated with persistent epigenome-wide disruptions despite smoking cessation.

Ana I Hernandez Cordero, Xuan Li, Chen Xi Yang, Amirtha Ambalavanan, Julie L MacIsaac, Michael S Kobor, Dany Doiron, Wan Tan, Jean Bourbeau, Don D Sin and 3 more

Registry-linked trialAbstract readObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in BMC pulmonary medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00920348 (The Canadian Cohort Obstructive Lung Disease), which is not on this map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT00920348 unknown statusnot on this map

The Canadian Cohort Obstructive Lung Disease

TypeobservationalSponsorMcGill University Health Centre/Research Institute of the McGill University Health CentreRan2010 to 2022Enrolled1,500ConditionsCOPD, Chronic Obstructive Pulmonary Disease
3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Ana I Hernandez CorderoCentre for Heart Lung Innovation, St. Paul'S Hospital and University of British Columbia, Room 166, 1081 Burrard Street, Vancouver, BC, V6Z 1Y6, Canada.
Xuan LiCentre for Heart Lung Innovation, St. Paul'S Hospital and University of British Columbia, Room 166, 1081 Burrard Street, Vancouver, BC, V6Z 1Y6, Canada.
Chen Xi YangCentre for Heart Lung Innovation, St. Paul'S Hospital and University of British Columbia, Room 166, 1081 Burrard Street, Vancouver, BC, V6Z 1Y6, Canada.
Amirtha AmbalavananDepartment of Biomedical and Molecular Sciences, School of Medicine, Queen's University, Kingston, Canada.
Julie L MacIsaacEdwin S. H. Leong Healthy Aging Program, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Michael S KoborEdwin S. H. Leong Healthy Aging Program, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Dany DoironMcGill University Health Centre, McGill University, Montreal, Canada.
Wan TanCentre for Heart Lung Innovation, St. Paul'S Hospital and University of British Columbia, Room 166, 1081 Burrard Street, Vancouver, BC, V6Z 1Y6, Canada.
Jean BourbeauMcGill University Health Centre, McGill University, Montreal, Canada.
Don D SinCentre for Heart Lung Innovation, St. Paul'S Hospital and University of British Columbia, Room 166, 1081 Burrard Street, Vancouver, BC, V6Z 1Y6, Canada.
Qingling Duan *Department of Biomedical and Molecular Sciences, School of Medicine, Queen's University, Kingston, Canada.
Janice M Leung *Centre for Heart Lung Innovation, St. Paul'S Hospital and University of British Columbia, Room 166, 1081 Burrard Street, Vancouver, BC, V6Z 1Y6, Canada. Janice.Leung@hli.ubc.ca.
CanCOLD Collaborative Research Group

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe use of cannabis has been associated with both therapeutic and harmful effects. As with cigarette smoking, cannabis smoking may affect the epigenetic regulation (e.g., DNA methylation) of gene expression which could result in long term health effects. The study of DNA methylation in cannabis smoking has to date been restricted to young adults and there remains yet no evaluation of whether cannabis smoking cessation can reverse epigenetic disturbances. Here, we aimed to investigate the relationship between genome-wide DNA methylation and cannabis smoking.

methodsWe used peripheral blood from a subset of older adults within the Canadian Cohort of Obstructive Lung Disease (CanCOLD) cohort (n = 93) to conduct an epigenome-wide DNA methylation analysis that identified differential methylated positions (DMPs) associated with cannabis smoking at a false discovery rate < 0.05. Using these DMPs, we then identified differentially methylated genes (DMGs) that enriched pathways associated with both former and current cannabis smoking status.

resultsWe found DMPs corresponding to 12,115 DMGs and 10,806 DMGs that distinguished the current and former cannabis smoking groups, respectively, from the never cannabis smoking group. 5,915 of these DMGs were shared between the current and former cannabis smoking groups. 50 enriched pathways were also shared between the current and former cannabis smoking groups, which were heavily represented by multiple aging- and cancer-related pathways.

conclusionsOur findings indicate that in older adults, cannabis smoking is linked with epigenome-wide disruptions, many of which persist despite cannabis smoking cessation. Epigenetic modulation of genes associated with aging and cancer that remains even after quitting cannabis should serve as a caution that there may be long-lasting epigenetic injury with cannabis smoking.

trial registrationNCT00920348.

Indexed as

DNA MethylationEpigenesis, GeneticEpigenomeMarijuana SmokingSmoking CessationAgedCanadaFemaleGenome-Wide Association StudyHumansMaleMiddle AgedCannabisEpigeneticsMarijuanaMethylationSmoking cessation

Identifiers

PMID40205553
PMCPMC11980083

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.