Evidence map›Paper›PMID 40204935›Full record

ArticleAnnals of hematology2025

Role of IRF4 in mediating plasmablast differentiation in diffuse large B-cell lymphomas via mTORC1 pathway.

Jingjing Gao, Chuntuan Li, Xingzhi Lin, Yanling Zhuang, Mingquan Wang, Hongjun Lin, Xiongpeng Zhu

Abstract read
PubMed Full text at PMC Publisher
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Article in Annals of hematology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jingjing GaoDepartment of Blood transfusion, Quanzhou First Hospital affiliated to Fujian Medical University, Quanzhou, Fujian, 362000, China.
Chuntuan LiDepartment of Hematology, Quanzhou First Hospital affiliated to Fujian Medical University, Quanzhou, Fujian, 362000, China.
Xingzhi LinDepartment of Blood transfusion, Quanzhou First Hospital affiliated to Fujian Medical University, Quanzhou, Fujian, 362000, China.
Yanling ZhuangDepartment of Blood transfusion, Quanzhou First Hospital affiliated to Fujian Medical University, Quanzhou, Fujian, 362000, China.
Mingquan WangDepartment of Blood transfusion, Quanzhou First Hospital affiliated to Fujian Medical University, Quanzhou, Fujian, 362000, China.
Hongjun LinMedical College of Huaqiao University, Quanzhou, Fujian, 362000, China.
Xiongpeng ZhuDepartment of Hematology, Quanzhou First Hospital affiliated to Fujian Medical University, Quanzhou, Fujian, 362000, China. xiongpengzhu@163.com.

Funding

Fujian Provincial Natural Science Foundation 2023J011785Quanzhou City Science and Technology Project 2021N057S
6 · The paper itself

Abstract

Autoimmune haemolytic anaemia (AIHA) is common secondary to diffuse large B-cell lymphoma (DLBCL). However, there are no reports on tumour B-cells differentiating into plasmablasts in DLBCL secondary AIHA. To state impact of the interferon regulatory factor 4 (IRF4) on DLBCL and explore the mechanism of IRF4 on plasmablast differentiation.We analysed the expression of immunity-related genes from the Gene Expression Omnibus and correlated predictors from clinical and laboratory data using R package and various statistical tools. Western blotting (WB) was used to detect protein levels in DLBCL cell lines of different subtypes to investigate the plasmablast and activation of mTORC1. To furtherly validate mTOR regulation of plasmablast differentiation, mTOR-activated and -inhibited cell models were constructed by CCK8 and flow cytometry (FCM) was used to assess the proportion of CD38 positive cells. We found that IRF4 was highly expressed in activated B-cell-like (ABC) DLBCL vs. germinal centre B-cell-like (GCB) DLBCL. Positive MUM-1and low haemoglobin values were corrected to non-CGB patients. Plasmablast indictors (BLIMP-1, ARF4, IRE1α, and IRF4) and mTORC1 pathway indictors (mTOR, p70S6K and phosphorylated-p70S6K) were different level in ABC cell lines. After successfully constructing cell models, the proportion of CD38

Indexed as

Cell DifferentiationInterferon Regulatory FactorsLymphoma, Large B-Cell, DiffuseMechanistic Target of Rapamycin Complex 1Neoplasm ProteinsPlasma CellsSignal TransductionCell Line, TumorFemaleGene Expression Regulation, NeoplasticHumansInterferon Regulatory Factor-4MaleMiddle AgedInterferon Regulatory Factor-4Interferon Regulatory FactorsMechanistic Target of Rapamycin Complex 1Neoplasm ProteinsDLBCLIRF4mTORPlasmablast differentiation

Identifiers

PMID40204935
PMCPMC12053343

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.