ArticleG3 (Bethesda, Md.)2025
Promising high fidelity genetic markers for sexing Cannabis sativa seedlings.
Article in G3 (Bethesda, Md.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Genetic Advances inPlants (Basel, Switzerland) · 2026Review
- CRISP: A cost-efficient, high-throughput pipeline for sex-identification inmicroPublication biology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The uses of Cannabis sativa, a dioecious species with an XY sex chromosome system, are varying from fiber and oil to cannabinoids, among others. In most cases, males are undesirable, and the sexual dimorphism at immature plants is too subtle for reliable phenotypic sexing, making genetic approaches promising. In this technical note, we present a multiplex PCR mix that includes 2 markers of Y-specific coding regions and 1 autosomal control marker. This PCR mix, tested across 12 hemp-type cultivars, encompassing approximately 200 individuals, achieved a 99.5% true positive rate in identifying the sex of C. sativa samples. Despite being tested on a limited number of germplasm, we believe that these markers offer a promising solution for sexing a larger diversity of C. sativa seedlings.
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Registered trials
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