Evidence map›Paper›PMID 40202844›Full record

ArticleCell reports2025

Antagonistic response regulators spatially regulate receptor methylation in the Pseudomonas aeruginosa Pil-Chp surface sensing system.

Ramiro Patino, Marco J Kühn, Henriette Macmillan, Yuki F Inclan, Ivan Chavez, Alexandre Persat, Joanne N Engel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Transcriptional profiling ofFrontiers in microbiology · 2026
    Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ramiro PatinoDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94158, USA.
Marco J KühnInstitute of Bioengineering and Global Health Institute, School of Life Sciences, EPFL, 1015 Lausanne, Switzerland.
Henriette MacmillanDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94158, USA.
Yuki F InclanDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94158, USA.
Ivan ChavezDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94158, USA.
Alexandre PersatInstitute of Bioengineering and Global Health Institute, School of Life Sciences, EPFL, 1015 Lausanne, Switzerland. Electronic address: alexandre.persat@epfl.ch.
Joanne N EngelDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94158, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: joanne.engel@ucsf.edu.

Funding

Finding the way: Sensory adaptation during bacterial mechanotransductionR01AI174014 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Joanne N. Engel · 2023 to 2026
$2.9M
Adapting to a changing environment: How surface contact induces virulence factor production in Pseudomonas aeruginosaR01AI129547 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ENGEL, JOANNE N. · 2017 to 2020
$1.6M
Understanding the role of sensory adaptation in bacterial mechanochemical signaling pathwaysR21AI154350 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ENGEL, JOANNE N. · 2020 to 2021
$444k
Investigating the role of conserved signaling modules in medically important Acinetobacter speciesF31AI147544 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI PATINO, RAMIRO · 2019 to 2022
$154k
NIAID NIH HHS F31 AI147544NIAID NIH HHS R01 AI129547NIAID NIH HHS R01 AI174014NIAID NIH HHS R21 AI154350
6 · The paper itself

Abstract

Most bacterial chemosensory systems encode enzymes that are predicted to methylate and demethylate their chemoreceptors to control signaling activity. Here, we show that a predicted methyltransferase (PilK) and methylesterase (ChpB) methylate and demethylate, respectively, the chemoreceptor PilJ in the Pseudomonas aeruginosa (P. aeruginosa) Pil-Chp surface sensing system. PilJ methylation modulates the amplitude of cAMP production and the frequency of twitching motility reversals, outputs of Pil-Chp signaling. Dynamic imaging of fluorescent fusion proteins in individual twitching cells reveals that PilK and ChpB localize to opposite poles, with PilK at the lagging pole and ChpB at the leading pole. These enzymes switch poles upon bacterial reversals, a process dependent on the Pil-Chp response regulators PilG and PilH. Our results suggest that the PilJ population at each pole exists in dynamic and opposite methylation states that may reset the PilJ signaling activity at each pole during P. aeruginosa surface sensing, a key feature of adaptation.

Indexed as

Bacterial ProteinsPseudomonas aeruginosaCyclic AMPMethylationMethyltransferasesSignal TransductionBacterial ProteinsCyclic AMPMethyltransferasesbacteriachemoreceptor methylationchemosensory systemCP: Microbiologymechanosensingpolar localizationPseudomonas aeruginosasensory adaptationsignal transductionsurface sensing

Identifiers

PMID40202844
PMCPMC12129077

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.