Evidence map›Paper›PMID 40202668›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Seminal Fluid Interaction with Female Reproductive Tract Epithelial Cells: In Vitro Models for Human.

David J Sharkey, Hannah E Lyons, Hon Yeung Chan, Sarah A Robertson

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

David J SharkeyRobinson Research Institute and School of Biomedicine, The University of Adelaide, Adelaide, SA, Australia. david.sharkey@adelaide.edu.au.
Hannah E LyonsRobinson Research Institute and School of Biomedicine, The University of Adelaide, Adelaide, SA, Australia.
Hon Yeung ChanRobinson Research Institute and School of Biomedicine, The University of Adelaide, Adelaide, SA, Australia.
Sarah A RobertsonRobinson Research Institute and School of Biomedicine, The University of Adelaide, Adelaide, SA, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Seminal fluid introduced into the female reproductive tract following unprotected coitus initiates an inflammation-like response in the cervical tissues that stimulates immune tolerance to male seminal fluid alloantigens and promotes immune adaptation for pregnancy. This response commences when seminal fluid factors induce genes encoding pro-inflammatory cytokines and chemokines including CSF2, IL6, and CXCL8, that in turn cause recruitment of macrophages, dendritic cells, and T cells into the epithelial and stromal layers. Various signaling agents present in seminal plasma provoke the post-coital pro-inflammatory activation and regulate the quality of immune response. However, the factors identified to date do not account for all the biological activity within seminal fluid, implying sperm-associated factors may also contribute. In this chapter, we report methods for evaluating the effects of human seminal fluid components (seminal plasma and sperm) on the female reproductive tract immune response in primary and immortalized ectocervical epithelial cells in vitro.

Indexed as

Epithelial CellsGenitalia, FemaleSemenCervix UteriCytokinesFemaleHumansMaleSpermatozoaCytokinesCervixCytokinesHumanInflammationSeminal fluidSeminal plasmaSperm

Identifiers

PMID40202668

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.