Evidence map›Paper›PMID 40202659›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Reduced Representation Bisulfite Sequencing Library Preparation for Sperm DNA Methylation Analyses: Manual and Automated Methods.

Jean-Philippe Perrier, Aurélie Chaulot-Talmon, Francesca Ali, Laurent Schibler, Hélène Jammes, Hélène Kiefer

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jean-Philippe Perrier *Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy en-Josas, France.
Aurélie Chaulot-Talmon *Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy en-Josas, France.
Francesca AliUniversité Paris-Saclay, UVSQ, INRAE, BREED, Jouy en-Josas, France.
Laurent SchiblerR&D Department, ALLICE, Paris, France.
Hélène JammesUniversité Paris-Saclay, UVSQ, INRAE, BREED, Jouy en-Josas, France.
Hélène KieferUniversité Paris-Saclay, UVSQ, INRAE, BREED, Jouy en-Josas, France. helene.kiefer@inrae.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Among epigenetic modifications, DNA methylation is extensively studied in sperm, and variations in sperm DNA methylation patterns have been associated with fertility, sperm quality, and in vitro fertilization outcomes in several species. Reduced representation bisulfite sequencing (RRBS) is a cost-effective method that has been successfully applied to sperm. However, RRBS library preparation remains sensitive and labor-intensive and can be subjected to diverse sources of technical variation. In order to improve RRBS library preparation performance, as well as its reproducibility, we adapted the reference protocol and implemented it on a Hamilton pipetting automaton.

Indexed as

DNA MethylationGene LibraryHigh-Throughput Nucleotide SequencingSequence Analysis, DNASpermatozoaSulfitesEpigenesis, GeneticHumansMalehydrogen sulfiteSulfitesAutomationDNA methylationGenome-wide epigenetic analysisHigh-throughputReduced representation bisulfite sequencingSperm

Identifiers

PMID40202659

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.