ArticleMethods in molecular biology (Clifton, N.J.)2025
Analysis of the Chromatin Structure by Chromomycin A3 (CMA3) and Flow Cytometry.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Dynamic architecture of mammalian paternal chromatin: histone-to-protamine exchange and post-fertilization reprogramming.Epigenetics & chromatin · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sperm chromatin compaction state refers to the tightly packaged structure in which the sperm DNA is specifically organized, unlike other cell types. The high grade of compaction in the sperm nucleus is crucial for preserving the integrity of the paternal genome during the journey from the testicle to the fertilization sites in many mammal species. The chromatin compaction results from complex transformations during spermiogenesis, in which protamines replace histones increasing DNA compaction. Chromomycin A3 (CMA3) staining is an indirect method to evaluate the sperm chromatin structure. An excess of histones (protamination defects) or a lack of disulfide cross-link causes an increase in the CMA3 fluorescence. Evaluated by flow cytometry, CMA3 staining enables quick detection of defects in the chromatin structure compaction. In this chapter, we described the method and some adaptations to perform this technique in mammal spermatozoa.
Indexed as
Identifiers
40202656What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.