ArticleMethods in molecular biology (Clifton, N.J.)2025
Real-Time Acrosome Reaction and Intracellular Calcium Recordings in Mice Sperm.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- The Sperm Acrosomal Reaction Depends on the Function of Both Na,K-ATPase α1 and α4 Isoforms.International journal of molecular sciences · 2026Article
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Authors and funding
4 authors.
Funding
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Abstract
Accurate determinations of acrosome reaction (AR) are fundamental to obtain physiologically relevant information of this essential event for sperm fertilization. For decades, the AR was exclusively studied in fixed preparation; more recently, new methods were developed to evaluate the AR in live sperm and in real time. These new technologies involved the use of genetically modified mice models, dyes with ion affinity, and fluorescent microscopy. In addition, these techniques allowed the reproduction field the possibility to follow the AR directly in the female tract under physiological conditions. Despite these advances, maintaining transgenic colonies is expensive for most research laboratories. Here, we present a methodology which can be mounted in a simple epifluorescence microscopy set in combination with appropriate filters enabling to follow the AR in real time. The implemented technique allows also to follow the AR in combination with [Ca
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