Evidence map›Paper›PMID 40202649›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Evaluation of Oxidative Stress in Mammalian Spermatozoa.

Zamira Gibb, Róisín A Griffin, Elizabeth G Bromfield, Sarah R Lambourne, Alexandra Wilkins, Ceilidh Jenkins, Aleona Swegen, R John Aitken, Geoffry N De Iuliis

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zamira GibbSchool of Environmental and Life Sciences, College of Engineering, Science and the Environment, The University of Newcastle, Callaghan, NSW, Australia. zamira.gibb@newcastle.edu.au.
Róisín A GriffinSchool of Environmental and Life Sciences, College of Engineering, Science and the Environment, The University of Newcastle, Callaghan, NSW, Australia.
Elizabeth G BromfieldSchool of Environmental and Life Sciences, College of Engineering, Science and the Environment, The University of Newcastle, Callaghan, NSW, Australia.
Sarah R LambourneSchool of Environmental and Life Sciences, College of Engineering, Science and the Environment, The University of Newcastle, Callaghan, NSW, Australia.
Alexandra WilkinsSchool of Environmental and Life Sciences, College of Engineering, Science and the Environment, The University of Newcastle, Callaghan, NSW, Australia.
Ceilidh JenkinsSchool of Environmental and Life Sciences, College of Engineering, Science and the Environment, The University of Newcastle, Callaghan, NSW, Australia.
Aleona SwegenSchool of Environmental and Life Sciences, College of Engineering, Science and the Environment, The University of Newcastle, Callaghan, NSW, Australia.
R John AitkenSchool of Environmental and Life Sciences, College of Engineering, Science and the Environment, The University of Newcastle, Callaghan, NSW, Australia.
Geoffry N De IuliisSchool of Environmental and Life Sciences, College of Engineering, Science and the Environment, The University of Newcastle, Callaghan, NSW, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oxidative stress, characterized by an imbalance between prooxidants and antioxidants in favor of the former, can lead to cellular damage due to the accumulation of reactive oxygen species (ROS) and reactive nitrogen species (RNS). In spermatozoa, oxidative stress plays a crucial role in signaling pathways associated with capacitation, a process essential for fertilization. However, the limited capacity of spermatozoa to buffer ROS makes them susceptible to oxidative stress. Measurement of oxidative stress in spermatozoa is important as it precedes or directly causes the loss of basic sperm functions-such as motility and the membrane changes required for zona binding and gamete fusion-associated with fertility. This chapter aims to describe protocols for assessing oxidative damage in spermatozoa, encompassing the generation of ROS in mitochondria to the manifestation of oxidative damage in DNA, proteins, and lipids. In addition to the detailed instructions provided within this chapter, we have sought to provide background information on the development and significance of the assays which we most commonly utilize in our laboratory.

Indexed as

Oxidative StressSpermatozoaAnimalsDNA DamageMaleMiceMitochondriaReactive Oxygen SpeciesSperm MotilityReactive Oxygen SpeciesCellular damageDNA damageFlow cytometryOxidative stressReactive oxygen speciesSpermatozoa

Identifiers

PMID40202649

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.