ArticleMethods in molecular biology (Clifton, N.J.)2025
Oxidative Status Measurement in Domestic and Wild Small Ruminants' Species.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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12 authors.
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Abstract
In sperm, oxidative stress triggers structural damage to biomolecules, DNA, lipids, carbohydrates, and proteins, as well as other cellular components that eventually impair multiple signaling pathways resulting in male sub/infertility. In this chapter, we describe the methods applied to evaluate the effects of the oxidative stress on sperm attributes of domestic and wild small ruminants' species, mainly by flow cytometry. Together with the traditional usage of fluorescent probes, we provide details of the knowhows involving the combination of immunodetection and the flow cytometry technologies.
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