Evidence map›Paper›PMID 40200176›Full record

ArticleBMC microbiology2025

Genomic and molecular characterization of a ceftazidime-avibactam resistant Klebsiella pneumoniae strain isolated from a Chinese tertiary hospital.

Yanye Tu, Hui Gao, Feng Wang, Rongqing Zhao, Shiyong Chen, Yanzi Chang, Hong Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yanye TuDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital, Ningbo University, Ningbo, 315000, China.
Hui GaoDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital, Ningbo University, Ningbo, 315000, China. gaoh711@126.com.
Feng WangDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital, Ningbo University, Ningbo, 315000, China.
Rongqing ZhaoDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital, Ningbo University, Ningbo, 315000, China.
Shiyong ChenDepartment of Hospital-acquired Infection Control, The Affiliated Li Huili Hospital, Ningbo University, Ningbo, 315000, China.
Yanzi ChangDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital, Ningbo University, Ningbo, 315000, China.
Hong LiDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital, Ningbo University, Ningbo, 315000, China. lancet2017@163.com.

Funding

Huili Fund 2022FZ003Natural Science Foundation of Ningbo in Zhejiang Province No. 2022J255Ningbo Major Research and Development Plan Project 2024Z215Zhejiang Medical and Health Science and Technology Plan NO.2023KY1042
6 · The paper itself

Abstract

objectiveThis study aims to investigate the molecular biological characteristics of a ceftazidime-avibactam resistant Klebsiella pneumoniae strain isolated from a tertiary care hospital in China.

methodsThe K. pneumoniae strain KP217360, isolated from a urine specimen at Li Huili Hospital affiliated with Ningbo University, was analyzed for antimicrobial susceptibility using the VITEK-2 Compact automated system. Antimicrobial susceptibility testing for polymyxin, tigecycline, and ceftazidime-avibactam was performed using broth microdilution according to CLSI guidelines. Whole genome sequencing was conducted using the Illumina platform, followed by comprehensive genomic analysis to characterize resistance determinants and virulence-associated genes. Experiments on conjugation were conducted to evaluate the transmissibility of the resistance plasmids. The virulence potential was evaluated through in vivo experiments using Galleria mellonella larvae as an infection model.

resultsThe K. pneumoniae KP217360 strain demonstrated extensive antimicrobial resistance, showing non-susceptibility to imipenem, ertapenem and ceftazidime-avibactam. Genomic characterization revealed the strain belonged to capsular type KL16 and harbored multiple resistance determinants, including bla

conclusionThis study reports the first identification of a ceftazidime-avibactam resistant K. pneumoniae ST685 strain carrying bla

Indexed as

Anti-Bacterial AgentsAzabicyclo CompoundsCeftazidimeDrug Resistance, Multiple, BacterialKlebsiella InfectionsKlebsiella pneumoniaeAnimalsbeta-LactamasesChinaDisease Models, AnimalDrug CombinationsGenome, BacterialHumansLarvaMicrobial Sensitivity TestsMothsAnti-Bacterial Agentsavibactam, ceftazidime drug combinationAzabicyclo Compoundsbeta-LactamasesCeftazidimeDrug CombinationsVirulence FactorsAntibiotic resistanceCeftazidime-AvibactamIncX3 type plasmidInfection controlKlebsiella pneumoniaeResistance genesSurveillance

Identifiers

PMID40200176
PMCPMC11978194

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.