Evidence map›Paper›PMID 40200137›Full record

ArticleBMC immunology2025

Fecal microbiota transplantation enhanced the effect of chemoimmunotherapy by restoring intestinal microbiota in LLC tumor-bearing mice.

Xinmeng Wang, Qian Geng, Hua Jiang, Jingyan Yue, Chunjian Qi, Lanqun Qin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Review
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  6. Article
  7. Article
  8. Microbiota and Microbiome.Progress in molecular and subcellular biology · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xinmeng Wang *Department of Oncology, Changzhou No.2 People's Hospital, the Third Affiliated Hospital of Nanjing Medical University, Changzhou, 213000, China.
Qian Geng *Department of Oncology, Changzhou No.2 People's Hospital, the Third Affiliated Hospital of Nanjing Medical University, Changzhou, 213000, China.
Hua JiangDepartment of Oncology, Changzhou No.2 People's Hospital, the Third Affiliated Hospital of Nanjing Medical University, Changzhou, 213000, China.
Jingyan YueDepartment of Oncology, Changzhou No.2 People's Hospital, the Third Affiliated Hospital of Nanjing Medical University, Changzhou, 213000, China.
Chunjian QiDepartment of Oncology, Changzhou No.2 People's Hospital, the Third Affiliated Hospital of Nanjing Medical University, Changzhou, 213000, China.
Lanqun QinDepartment of Oncology, Changzhou No.2 People's Hospital, the Third Affiliated Hospital of Nanjing Medical University, Changzhou, 213000, China. 1103291776@qq.com.

Funding

Changzhou 8th Batch of Science and Technology Project (Applied Basic Research) CJ20220086Clinical Research Project of Changzhou Medical Center, Nanjing Medical University CMCC202201
6 · The paper itself

Abstract

objectiveTo assess the effect of half-dose chemotherapy (HDC) and standard-dose chemotherapy (SDC) on the intestinal microbiota and to investigate whether fecal microbiota transplantation (FMT) can restore the intestinal microecology to enhance the efficacy of chemoimmunotherapy containing an anti-PD- 1 antibody (PD1).

methodsLewis lung cancer (LLC) tumor-bearing mice were divided into six groups, including Control, HDC, SDC, SDC + FMT, SDC + PD1, and SDC + PD1 + FMT. After the treatment, analyses were conducted on intestinal microbiota using 16S rRNA sequencing, immune cells through flow cytometry, cytokines and chemokines via polymerase chain reaction (PCR), and programmed death-ligand 1 (PD-L1) expression in tumor tissues by immunohistochemistry.

resultsAlpha and beta diversity of intestinal flora were not significantly different between HDC and SDC groups, nor was there a significant difference in the abundance of the top 10 species at the phylum, class, order, family, genus, or species levels. FMT increased both alpha and beta diversity and led to an increase in the abundance of Ruminococcus_callidus and Alistipes_finegoldii at the species level in mice receiving SDC + FMT. Besides, tumor growth was significantly slowed in SDC + PD1 + FMT compared to SDC + PD1 group, accompanied by an up-regulated Bacteroidetes/Firmicutes ratio, down-regulated abundance of Proteobacteria species (including Pseudolabrys, Comamonas, Alcaligenaceae, Xanthobacteraceae and Comamonadaceae), as well as Faecalicoccus of Firmicutes, the increased number of cDC1 cells, cDC2 cells, CD4

conclusionsThere were no significant differences between HDC and SDC in their effects on the intestinal microbiota. FMT exhibited a beneficial impact on gut microbiota and improved the efficacy of chemoimmunotherapy, possibly associated with the increase of immune cells and the modulation of related cytokines and chemokines.

Indexed as

Carcinoma, Lewis LungFecal Microbiota TransplantationGastrointestinal MicrobiomeImmunotherapyAnimalsMiceMice, Inbred C57BLProgrammed Cell Death 1 ReceptorRNA, Ribosomal, 16SProgrammed Cell Death 1 ReceptorRNA, Ribosomal, 16SChemoimmunotherapyFecal microbiota transplantationHalf-dose chemotherapyImmune checkpoint inhibitorsIntestinal microecology

Identifiers

PMID40200137
PMCPMC11978186

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.