Sequencing Trait-Associated Mutations (STAM) to Clone Rust Resistance Genes.

Fei Ni, Yang Yu, Lynn Epstein, Daolin Fu, Jiajie Wu

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Fei NiState Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an, Shandong, China.

Yang YuState Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an, Shandong, China.

Lynn EpsteinDepartment of Plant Pathology, University of California, Davis, CA, USA.

Daolin FuState Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an, Shandong, China. dlfu@sdau.edu.cn.

Jiajie WuState Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an, Shandong, China. jiajiewu@sdau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sequencing trait-associated mutations (STAM) is a simple and straightforward gene cloning method that was developed in wheat. It uses full-length isoform sequencing (Iso-Seq) of the wild type as the reference and employs transcriptome sequencing of multiple, independently derived mutants for gene cloning. The STAM method eliminates the need for fine-mapping or a high-quality whole genome assembly of a specific wheat cultivar, and it could also be used in other plant species with complex genomes. Detailed, bioinformatic analysis protocol and tips for STAM are provided in this chapter.

Indexed as

Cloning, MolecularDisease ResistanceMutationPlant DiseasesTriticumComputational BiologyGenes, PlantGenome, PlantEthyl methanesulfonate (EMS)Gene cloningIso-SeqMutagenesisResistance geneRNA-SeqSTAMStripe rust

Identifiers

PMID40198565

DOI10.1038/s41467-022-29132-8

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.