ArticleMikrochimica acta2025
A self-calibrated tri-emission fluorescent probe based on carbon dots assembly for in vitro pepsin detection.
Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A novel self-calibrating triple-emission fluorescent probe (CDs/BSA-SQ-Cy7) was engineered through the strategic integration of blue-emitting carbon dots (CDs) with covalently assembled near-infrared fluorescent dyes (BSA-SQ and BSA-SQ-Cy7) for accurate in vitro pepsin detection. To validate the enhanced performance of the tri-emissive design, a dual-emission control probe (CDs/BSA-SQ) was developed. BSA-SQ-Cy7 undergoes hydrolysis upon interaction with pepsin, resulting in concentration-dependent quenching of the red fluorescence, where the blue CDs serve as a reference emission during the process. The CDs/BSA-SQ-Cy7 probe demonstrated a robust linear correlation with pepsin concentrations ranging from 0.018 to 30 μg/mL (determined by the lowest detection limit). The detection limits were found to be 0.182 μg/mL (I
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