ArticlePlant cell reports2025
Double-truncated version of OsGADs leads to higher GABA accumulation and stronger stress tolerance in Oryza sativa L. var. japonica.
Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Rice Quality: A Multidimensional Evaluation Integrating Ecology, Management and Genetic Regulation.Foods (Basel, Switzerland) · 2026Review
- Regulatory networks and molecular mechanisms underlying salt stress tolerance in rice.Frontiers in plant science · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
key messageCalmodulin binding domain truncation from OsGAD1 and OsGAD3 resulted in enhanced GABA accumulation, upregulated stress related genes, and improved tolerance to multiple abiotic stresses. Rice (Oryza sativa L.), a critical crop for global food security, faces significant challenges from abiotic stresses. Gamma-aminobutyric acid (GABA), synthesized by glutamate decarboxylase (GAD), plays a vital role in stress tolerance. Truncating the calmodulin-binding domain (CaMBD) in GAD enzymes enhances GAD activity and GABA production. In this study, we developed a hybrid line, Hybrid #78, by crossing two genome-edited lines, OsGAD1ΔC #5 and OsGAD3ΔC #8, with truncated CaMBD in OsGAD1 and OsGAD3, respectively. Hybrid #78 demonstrated significantly improved survival rates in cold (25%), salinity (33%), flooding (83%), and drought (83%) stress conditions, compared with wild-type Nipponbare (0-33%), OsGAD1∆C #5 (0-66%), and OsGAD3∆C #8 (0-50%). Hybrid #78 showed the highest GABA levels during stress, with increases of 3.5-fold (cold), 3.9-fold (salinity), 5-fold (flooding), and 5-fold (drought) relative to wild-type Nipponbare and up to 2-fold higher than that of the parent lines. RNA-seq analysis from shoot tissues in control conditions identified 975 differentially expressed genes between Hybrid #78 and wild-type Nipponbare, with 450 genes uniquely expressed in the hybrid. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that upregulation in nitrogen metabolism pathways likely contributes to enhanced GABA synthesis via increased glutamate production. Hybrid #78 also showed broader gene expression variability, suggesting enhanced adaptability to stress, especially upregulation of stress-related genes, such as OsDREB, OsHSP70, and OsNAC3. These findings highlight the potential of CaMBD truncation in OsGAD1 and OsGAD3 to develop rice lines with increased GABA accumulation and resilience to multiple abiotic stresses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.