Evidence map›Paper›PMID 40198376›Full record

ArticleApplied microbiology and biotechnology2025

Identification of chicken-derived antibodies targeting the Candida albicans Als3 protein.

Chi-Hsin Lee, Chao-Jung Wu, Fang-Yi Yen, Jia-Yun Chiang, Ting-Jing Shen, Sy-Jye Leu, Chuang-Rung Chang, Hsiu-Jung Lo, Bor-Yu Tsai, Yan-Chiao Mao and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. MixedJournal of fungi (Basel, Switzerland) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Chi-Hsin Lee *School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No. 301, Yuantong Rd., Zhonghe Dist., New Taipei City, 235, Taiwan.
Chao-Jung Wu *School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No. 301, Yuantong Rd., Zhonghe Dist., New Taipei City, 235, Taiwan.
Fang-Yi YenSchool of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No. 301, Yuantong Rd., Zhonghe Dist., New Taipei City, 235, Taiwan.
Jia-Yun ChiangSchool of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No. 301, Yuantong Rd., Zhonghe Dist., New Taipei City, 235, Taiwan.
Ting-Jing ShenSchool of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No. 301, Yuantong Rd., Zhonghe Dist., New Taipei City, 235, Taiwan.
Sy-Jye LeuDepartment of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 110301, Taiwan.
Chuang-Rung ChangInstitute of Biotechnology, National Tsing Hua University, Hsinchu, 300044, Taiwan.
Hsiu-Jung LoNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, 350401, Taiwan.
Bor-Yu TsaiNavi Bio-Therapeutics Inc., Taipei, 10351, Taiwan.
Yan-Chiao MaoDivision of Clinical Toxicology, Department of Emergency Medicine, Taichung Veterans General Hospital, Taichung, 407219, Taiwan.
Valencia AndrianiSchool of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No. 301, Yuantong Rd., Zhonghe Dist., New Taipei City, 235, Taiwan.
Priskila Cherisca ThenakaSchool of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No. 301, Yuantong Rd., Zhonghe Dist., New Taipei City, 235, Taiwan.
Wei-Chu WangPh.D. Program in Medical Biotechnology, College of Medical Science and Technology, Taipei Medical University Inc., Taipei, 110301, Taiwan.
Yu-Pin ChaoiReal Biotechnology Inc., Hsinchu, 30060, Taiwan.
Yi-Yuan YangSchool of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No. 301, Yuantong Rd., Zhonghe Dist., New Taipei City, 235, Taiwan. yangyuan@tmu.edu.tw.ORCID http://orcid.org/0000-0003-2534-8436

Funding

National Science and Technology Council MOST 109-2320-B-038-053National Science and Technology Council MOST 110-2320-B-075A-001
6 · The paper itself

Abstract

Candida albicans is a major opportunistic pathogen, responsible for nearly half of clinical candidemia cases. The rising prevalence of azole-resistant Candida species represents a significant clinical challenge, underscoring the urgent need for alternative therapeutic strategies. Monoclonal antibody-based therapies have emerged as a promising and cost-effective approach to combating Candida infections. Agglutinin-like sequence protein 3 (Als3), a key cell surface protein of C. albicans, plays a pivotal role in adherence and biofilm formation, both of which are essential for its pathogenesis. In this study, recombinant Als3 protein was purified and utilized to immunize chickens, resulting in the production of Als3-specific immunoglobulin Y (IgY) antibodies. Two single-chain variable fragment (scFv) antibody libraries were subsequently constructed using phage display technology, yielding transformant counts of 5.3 × 10

Indexed as

Antibodies, FungalCandida albicansFungal ProteinsSingle-Chain AntibodiesAnimalsCell Surface Display TechniquesChickensEnzyme-Linked Immunosorbent AssayImmunoglobulinsPeptide LibraryRecombinant ProteinsALS3 protein, Candida albicansAntibodies, FungalFungal ProteinsIgYImmunoglobulinsPeptide LibraryRecombinant ProteinsSingle-Chain AntibodiesAgglutinin-like sequence protein 3 (Als3)Candida albicansChicken IgYPhage displaySingle-chain antibody (scFv)

Identifiers

PMID40198376
PMCPMC11978541

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.