ArticleFrontiers in veterinary science2025
Establishment and application of multiplex PCR method for detection of
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- A DPO-Enhanced Gold Nanoparticle-Assisted PCR Assay for Simultaneous Detection of Bovine Viral Diarrhea Virus, Astrovirus, and Rotavirus.Animals : an open access journal from MDPI · 2026Article
- Molecular identification and mitogenomic characterization of cattle-associatedFrontiers in veterinary science · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Dermatophytosis, which is a contagious fungal skin infection common in animals and humans, is the most common skin disease in cattle. It has a serious negative impact on the livestock industry. In order to circumvent the shortcomings of traditional detection methods such as time-consuming and low isolation rate. Therefore, this study established a simple, rapid and effective diagnostic method to accurately diagnose and differentiate the causative fungi of dermatophytosis, which is of great significance to enhance the prevention and treatment of dermatophytosis in beef cattle farms. Methods: Three pairs of specific primers were designed using Primer Premier 5.0 from Results and discussion: In this study, a multiplex PCR method that can rapidly detect these three fungi at the same time was established, and its specificity, sensitivity and repeatability were analyzed at the same time. The results showed that the multiplex PCR method amplified the specific expected fragments of 581 bp, 1,513 bp and 371 bp for
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.