Evidence map›Paper›PMID 40196496›Full record

ArticlebioRxiv : the preprint server for biology2025

Structural basis of antiphage defense by an ATPase-associated reverse transcriptase.

Jerrin Thomas George, Nathaniel Burman, Royce A Wilkinson, Senuri de Silva, Quynh McKelvey-Pham, Murat Buyukyoruk, Adelaide Dale, Hannah Landman, Ava Graham, Steven Z DeLuca and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Jerrin Thomas GeorgeMontana State University, Bozeman, Department of Microbiology and Cell Biology, Bozeman, Montana 59717, USA.ORCID 0000-0001-9696-0564
Nathaniel BurmanMontana State University, Bozeman, Department of Microbiology and Cell Biology, Bozeman, Montana 59717, USA.ORCID 0000-0002-3732-3159
Royce A WilkinsonMontana State University, Bozeman, Department of Microbiology and Cell Biology, Bozeman, Montana 59717, USA.ORCID 0000-0001-8831-2081
Senuri de SilvaMontana State University, Bozeman, Department of Microbiology and Cell Biology, Bozeman, Montana 59717, USA.ORCID 0000-0002-9870-4449
Quynh McKelvey-PhamMontana State University, Bozeman, Department of Microbiology and Cell Biology, Bozeman, Montana 59717, USA.
Murat BuyukyorukMontana State University, Bozeman, Department of Microbiology and Cell Biology, Bozeman, Montana 59717, USA.ORCID 0000-0003-1534-7202
Adelaide DaleMontana State University, Bozeman, Department of Microbiology and Cell Biology, Bozeman, Montana 59717, USA.
Hannah LandmanMontana State University, Bozeman, Department of Microbiology and Cell Biology, Bozeman, Montana 59717, USA.ORCID 0009-0008-1666-8300
Ava GrahamMontana State University, Bozeman, Department of Microbiology and Cell Biology, Bozeman, Montana 59717, USA.ORCID 0000-0002-6782-5248
Steven Z DeLucaMontana State University, Bozeman, Department of Microbiology and Cell Biology, Bozeman, Montana 59717, USA.ORCID 0000-0003-0683-8413
Blake WiedenheftMontana State University, Bozeman, Department of Microbiology and Cell Biology, Bozeman, Montana 59717, USA.ORCID 0000-0001-9297-5304

Funding

Research Core (Developmental Research Project Program)P20GM103474 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Ann Therese Bertagnolli · 2012 to 2026
$60.0M
ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
Structural and functional understanding of bacterial defense and viral counter defenseR35GM134867 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Blake A Wiedenheft · 2020 to 2026
$3.8M
Developmental control of gene silencingR35GM151262 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Steven Deluca · 2023 to 2026
$1.7M
CRISPR RNA-guided recognition of invading RNA activates a multipronged cell death responseF31GM153146 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI BURMAN, NATHANIEL · 2024 to 2025
$73k
NIGMS NIH HHS F31 GM153146NIGMS NIH HHS P20 GM103474NIGMS NIH HHS R01 GM129325NIGMS NIH HHS R35 GM134867NIGMS NIH HHS R35 GM151262
6 · The paper itself

Abstract

Reverse transcriptases (RTs) have well-established roles in the replication and spread of retroviruses and retrotransposons. However, recent evidence suggests that RTs have been conscripted by cells for diverse roles in antiviral defense. Here we determine structures of a type I-A retron, which explain how RNA, DNA, RT, HNH-nuclease and four molecules of an SMC-family ATPase assemble into a 364 kDa complex that provides phage defense. We show that phage-encoded nucleases trigger degradation of the retron-associated DNA, leading to disassembly of the retron and activation of the HNH nuclease. The HNH nuclease cleaves tRNA

Identifiers

PMID40196496
PMCPMC11974896

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.