Evidence map›Paper›PMID 40195982›Full record

ArticleResearch square2025

A CRISPR-Cas9 System for Knock-out and Knock-in of High Molecular Weight DNA Enables Module-Swapping of the Pikromycin Synthase in its Native Host.

Zhe-Chong Wang, Hayden Stegall, Takeshi Miyazawa, Adrian T Keatinge-Clay

Abstract readPreprint
In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Zhe-Chong WangThe University of Texas at Austin.
Hayden StegallThe University of Texas at Austin.
Takeshi MiyazawaThe University of Texas at Austin.
Adrian T Keatinge-ClayThe University of Texas at Austin.

Funding

Harnessing Polyketide Assembly Lines for Medicinal ChemistryR01GM145992 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI KEATINGE-CLAY, ADRIAN TRISTAN · 2022 to 2025
$1.3M
NIGMS NIH HHS R01 GM145992
6 · The paper itself

Abstract

Background: Engineers seeking to generate natural product analogs through altering modular polyketide synthases (PKSs) face significant challenges when genomically editing large stretches of DNA. Results: We describe a CRISPR-Cas9 system that was employed to reprogram the PKS in Conclusion: Our gene-editing tool has enabled the efficient replacement of extensive and repetitive DNA regions within streptomycetes.

Indexed as

CRISPR/Cas9module-swappingpikromycinPKS engineeringriboswitchS. venezuelae ATCC 15439type I PKSsupdated module boundary

Identifiers

PMID40195982
PMCPMC11975015

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.