ArticleSmall (Weinheim an der Bergstrasse, Germany)2025
Enhancing Multi-Enzyme Cascade Activity in Metal-Organic Frameworks via Controlled Enzyme Encapsulation.
Article in Small (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Oppositely Charged Single Enzyme Nanogels Form Versatile Coacervates for Efficient Enzyme Cascade Catalysis.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Enhanced Enzyme Cascade Reactions Through Coencapsulation in Biocompatible Silica Nanoconfinement.Chembiochem : a European journal of chemical biology · 2026Article
- A hierarchical dual-lock mechanism empowered epifluidic patch for rapid glucose feedback in exercise-induced sweating.Mikrochimica acta · 2026Article
- Article
- Sequential Optimization of Multivariate Metal-Organic Framework Based Biocatalysis.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Recent Advances in Metal-Organic Framework-Based Nanozymes for Enhanced Biosensing Applications.Biosensors · 2026Review
- Nature-Inspired Enzymatic Cascades: Emerging Strategies for Sustainable Chemistry.Molecules (Basel, Switzerland) · 2026Review
- Multicomponent synergistic immobilization via in-situ and dynamic exchange strategies for constructing hierarchical biopharmaceuticals.Nature communications · 2026Article
- Enhancing Multi-Enzyme Cascade Activity in Metal-Organic Frameworks via Controlled Enzyme Encapsulation.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
To position multi-enzymes in a core-shell structure, the conventional layer-by-layer approach is often used. However, this method is time-consuming and complex, requiring multiple steps and the isolation of intermediates at each stage. To address this challenge, a sequential strategy is introduced for the controlled encapsulation of multi-enzymes within metal-organic frameworks (MOFs), achieving a core-shell structure without the need for intermediate isolation. Synchrotron Terahertz-Far-Infrared (THz-Far-IR) spectroscopy is employed to monitor this encapsulation process. The results revealed that the first enzyme is co-precipitated within the MOFs, followed by biomineralization upon the addition of a second enzyme, achieving distinct enzyme positioning. This approach is applicable to both two-enzyme and three-enzyme cascade systems. The results demonstrate that multi-enzyme cascade activity is significantly enhanced compared to conventional one-pot and layer-by-layer approaches, owing to optimal spatial arrangement, increased surface area, and improved enzyme conformation. Furthermore, the encapsulated enzymes exhibit strong resistance to high temperatures, proteolysis, and organic solvents, along with excellent reusability, making this method highly promising for industrial biocatalytic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.