Evidence map›Paper›PMID 40195375›Full record

ArticleScientific reports2025

Molecular detection, seasonality, epidemiology and effect of Brucella melitensis infection on the hematological profile of cattle breeds.

Babar Ali, Muhammad Naeem, Shakir Ullah, Hamza Manzoor, Muhammad Asif, Nasir Mahmood, Aliza Kanwal, Mohammed Bourhia, Musaab Dauelbait, Turki M Dawoud and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Babar Ali *Department of Zoology, Islamia University, Bahawalpur, 06314, Pakistan.
Muhammad Naeem *Institute of Zoology, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Shakir UllahDepartment of Zoology, Abdul Wali Khan University, Mardan, Pakistan.
Hamza ManzoorDepartment of Theriogenology, University of Veterinary and Animal Sciences Lahore, Lahore, 54600, Pakistan.
Muhammad AsifInstitute of Zoology, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Nasir MahmoodFaculty of Veterinary Sciences, Bahauddin Zakariya University Multan, Multan, 60800, Pakistan.
Aliza KanwalInstitute of Zoology, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Mohammed BourhiaSwalife Biotech Ltd Unit 3D North Point House, North Point Business Park, Cork, Ireland.
Musaab DauelbaitUniversity of Bahr el Ghazal, Freedowm Stree, 91113, Wau, South Sudan. musaabelnaim@gmail.com.
Turki M DawoudDepartment of Botany and Microbiology, College of Science, King Saud University, P. O. BOX 2455, 11451, Riyadh, Saudi Arabia.
Adil KhanDepartment of Botany and Zoology, Bacha Khan University, Charsadda, Khyber Pakhtunkhwa, 24420, Pakistan. dradilkhan@bkuc.edu.pk.
Ahmad AliDepartment of Zoology, Islamia University, Bahawalpur, 06314, Pakistan.
Furhan IqbalInstitute of Zoology, Bahauddin Zakariya University, Multan, 60800, Pakistan. furhan.iqbal@bzu.edu.pk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Brucellosis is a bacterial zoonosis caused by various Brucella species, affecting both animals and humans. The incidence is particularly high in tropical and subtropical regions that are known for higher tick density. Present study was designed to improve our knowledge regarding Brucella melitensis infection in Pakistani cattle. A total of 903 blood samples were collected. Three cattle breeds, Holstein Friesian (N = 298), Sahiwal (N = 299), and crossbred (N = 306), were enrolled along with epidemiological data during March 2022 till April 2023 from district Multan in Punjab province. A PCR-based approach targeting IS711 estimated an overall prevalence of 4% (39/903). Representative partial IS711 sequences of three Pakistani isolates revealed a single genotype that was similar to Brucella melitensis sequences reported from aborted fetus in China. All cattle breeds were equally susceptible to this infection. For Sahiwal breed, bacterial infection varied between sampling seasons. Epidemiological factor analysis revealed that farms where only cattle were reared and dogs at farm having tick load had higher Brucella melitensis infection rates. Red blood cells, lymphocyte (%), monocyte (%), mean cell volume, hematocrit, mean corpuscular hemoglobin concentration and platelet count were significantly disturbed in Brucella melitensis positive cattle of all three breeds. A longitudinal survey and phylogenetic positioning of Brucella melitensis is recommended for epidemiological correlation, diagnosis and treatment of brucellosis in Pakistan.

Indexed as

Brucella melitensisBrucellosisBrucellosis, BovineCattle DiseasesAnimalsCattlePakistanSeasonsBrucella melitensisCattle breedsMolecular prevalencePhylogenetic analysisRisk factors

Identifiers

PMID40195375
PMCPMC11977288

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.