ArticleNucleic acids research2025
Altering translation allows E. coli to overcome G-quadruplex stabilizers.
Rachel R Cueny, Andrew F Voter, Aidan M McKenzie, Marcel Morgenstern, Kevin S Myers, Michael M Place, Jason M Peters, Joshua J Coon, James L Keck
Abstract read
In one paragraphArticle in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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What it found
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2 · The registryThe trial behind it
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4 · The recordCorrections and comments
5 · Who and what moneyAuthors and funding
9 authors.
Rachel R CuenyBiomolecular Chemistry Department, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53706 United States.ORCID 0000-0002-7388-6864 Andrew F VoterBiomolecular Chemistry Department, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53706 United States.
Aidan M McKenzieBiomolecular Chemistry Department, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53706 United States.
Marcel MorgensternBiomolecular Chemistry Department, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53706 United States.
Kevin S MyersGreat Lakes Bioenergy Research Center and the Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, WI, 53726 United States.ORCID 0000-0003-3302-3877 Michael M PlaceGreat Lakes Bioenergy Research Center and the Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, WI, 53726 United States.
Jason M PetersPharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, Madison, WI, 53706 United States.
Joshua J CoonBiomolecular Chemistry Department, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53706 United States.
James L KeckBiomolecular Chemistry Department, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53706 United States.ORCID 0000-0002-5961-0220 Funding
TR&D 2 Metabolic Labels for Ultraplexed Protein Quantification p. 453P41GM108538 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI COON, JOSHUA J · 2016 to 2025
$13.1MStructure, Function and Regulation of the ProteomeR35GM118110 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI COON, JOSHUA J · 2016 to 2025
$9.1MStructure and Function of the Bacterial PrimosomeR01GM098885 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI James L Keck · 2012 to 2026
$4.0MThe critical roles of (p)ppGpp in homeostasis and antibiotic tolerance in Gram positive bacteriaR35GM127088 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Jue D. Wang · 2018 to 2026
$3.9MNIGMS NIH HHS P41 GM108538NIGMS NIH HHS R01 GM098885NIGMS NIH HHS R01GM098885NIGMS NIH HHS R35 GM118110NIGMS NIH HHS R35 GM127088
6 · The paper itselfAbstract
G-quadruplex (G4) structures can form in guanine-rich DNA or RNA and have been found to modulate cellular processes, including replication, transcription, and translation. Many studies on the cellular roles of G4s have focused on eukaryotic systems, with far fewer probing bacterial G4s. Using a chemical-genetic approach, we identified genes in Escherichia coli that are important for growth in G4-stabilizing conditions. Reducing levels of translation elongation factor Tu or slowing translation initiation or elongation with kasugamycin, chloramphenicol, or spectinomycin suppress the effects of G4-stabilizing compounds. In contrast, reducing the expression of specific translation termination or ribosome recycling proteins is detrimental to growth in G4-stabilizing conditions. Proteomic and transcriptomic analyses reveal decreased protein and transcript levels, respectively, for ribosome assembly factors and proteins associated with translation in the presence of G4 stabilizer. Our results support a model in which reducing the rate of translation by altering translation initiation, translation elongation, or ribosome assembly can compensate for G4-related stress in E. coli.
Indexed as
DNA, BacterialDNA Transposable ElementsEscherichia coliG-QuadruplexesCRISPR-Cas SystemsEscherichia coli ProteinsGene DeletionGene Expression Regulation, BacterialPeptide Elongation Factor TuProtein BiosynthesisProteomicsRibosomesDNA, BacterialDNA Transposable ElementsEscherichia coli ProteinsPeptide Elongation Factor TutufB protein, E coli
Identifiers
PMID40193707
PMCPMC11975287
What OpenQuestion holds
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