ArticleInternational ophthalmology2025
Wnt7a can upregulate cell adhesion and migration related genes expression and facilitate the repair of corneal epithelial cells after injury.
Article in International ophthalmology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Distinct transcriptional regulation of the Wnt and TGFβ signaling families associated with wound healing and fibrotic outcomes to lens injury.Experimental eye research · 2025Article
- Correspondence: CALR promotes corneal epithelial cell proliferation and migration through Wnt7a.Molecular biology reports · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
purposeTo investigate the role of Wnt7a in corneal epithelial repair and its underlying mechanisms.
methodsImmunohistochemistry and immunofluorescence were used to assess Wnt7a expression in mouse corneas under normal and injury conditions. Human corneal epithelial cells (HCECs) were treated with Wnt7a siRNA or recombinant human Wnt7a (rhWnt7a) to evaluate proliferation (CCK-8 assay) and migration (scratch assay). Transcriptome sequencing and western blotting were performed to identify Wnt7a-regulated pathways and proteins.
resultsUnder normal conditions, Wnt7a was predominantly localized to the corneal limbus basal cells. Following corneal injury, its expression significantly increased in central corneal epithelial cells and co-localized with nuclei during repair. Wnt7a siRNA suppressed HCEC proliferation and migration, while rhWnt7a enhanced proliferation. Transcriptome analysis revealed upregulation of cell adhesion-related genes (e.g., FN1, ITGBs, LAMs), particularly fibronectin (FN), validated by increased FN protein levels after rhWnt7a treatment. Pathway enrichment implicated PI3K/Akt, Wnt signaling, and ECM-receptor interactions.
conclusionWnt7a promotes corneal epithelial repair by enhancing migration and proliferation, primarily through upregulating fibronectin and ECM-related pathways. These findings highlight Wnt7a as a potential therapeutic target for accelerating corneal wound healing.
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Registered trials
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