Evidence map›Paper›PMID 40192839›Full record

ArticleMolecular biology reports2025

A diagnostic target in the 3' untranslated region of hepatitis C virus genome.

Weenassarin Ampoot, Suthipong Chujan, Anusorn Kiattanaphon, Anchalee Sistayanarain

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Weenassarin AmpootDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.
Suthipong ChujanDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.
Anusorn KiattanaphonDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.
Anchalee SistayanarainDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand. sisaya@rocketmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionHepatitis C virus (HCV) causes hepatitis worldwide. Detection of HCV infection is important for diagnosis and treatment. The most frequently used method for diagnosis is the detection of the 5' untranslated (5' UTR) conserved target using polymerase chain reaction (PCR). Unfortunately, the detection of the 5'UTR is not completely reliable. The 5'UTR may not be the best target for nucleic acid detection because of its complex secondary structures and the variations of different HCV strains. An alternative target is the 3'UTR. The purpose is to evaluate the diagnostic potential of the 3'UTR of HCV. METHODS AND

resultsRNA was extracted from sixty-three serum samples. The 3'UTR and 5'UTR were amplified. Within the fifty-nine anti-HCV-positive samples, targeting the HCV 3'UTR and 5'UTR resulted in amplification in 40 (67.8%) and 54 (91.5%) of the samples, respectively. Five samples that were not amplified by 5'UTR detection were found positive by 3'UTR detection.

conclusionThese results indicate that PCR detection of the HCV 3'UTR appears less effective than detection of the 5'UTR. Nevertheless, the detection of several regions of the HCV genome by multiplex PCR may improve the accuracy of HCV detection.

Indexed as

3' Untranslated RegionsGenome, ViralHepacivirusHepatitis C5' Untranslated RegionsHumansMultiplex Polymerase Chain ReactionPolymerase Chain ReactionRNA, Viral3' Untranslated Regions5' Untranslated RegionsRNA, Viral3’ untranslated region5’ untranslated regionHCV detectionHepatitis C virus (HCV)Reverse transcription-polymerase chain reaction (RT-PCR)

Identifiers

PMID40192839

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