ArticleAngewandte Chemie (International ed. in English)2025
A Single-Molecule Liposome Assay for Membrane Permeabilization.
Article in Angewandte Chemie (International ed. in English), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Dual-Channel Fluorescence Assays with Supramolecular Host-Dye Reporter Pairs for Membrane Activity Mapping of Peptides.Angewandte Chemie (International ed. in English) · 2026Article
- Fluorescence Characterization of Extracellular Vesicles Using Single-Molecule Confocal Microscopy.Small methods · 2025Article
- A Single-Molecule Liposome Assay for Membrane Permeabilization.Angewandte Chemie (International ed. in English) · 2025Article
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Authors and funding
10 authors.
Funding
Abstract
Cell membrane disruption is associated with numerous diseases and underlies the activity of various antimicrobial agents. The rapid screening of compounds capable of disrupting or permeabilizing biological membranes is essential to the search for new therapeutic drugs. Here, we present a single-molecule confocal microscopy assay integrated with fast-flow microfluidics to study membrane permeabilization in large unilamellar vesicles (LUVs) containing as few as seven dye molecules. This assay eliminates the need for liposome immobilization and achieves detection rates in the range of 1000 vesicles per minute, offering unparalleled sensitivity and detection limits as low as 135 pM, corresponding to just eight permeabilizing molecules per vesicle for active compounds such as ionomycin. It provides a robust platform for investigating membrane-disrupting agents, including those with antimicrobial properties or implicated in neurodegenerative diseases.
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Registered trials
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