ArticleACS omega2025
Resolving Peak Overlap in HPLC Analysis of Glycerol Oxidation Products by Utilizing Various Detectors: Application to BiVO
Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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9 authors.
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Abstract
Glycerol, often considered a waste byproduct of biodiesel production, holds the potential for conversion into chemicals of varying economic value, such as dihydroxyacetone (DHA) and formic acid (FA). Hence, accurate identification and quantification of glycerol oxidation reaction (GOR) products are crucial for glycerol valorization research and practical deployment. High-performance liquid chromatography (HPLC) is the preferred analytical method for these purposes due to its proficiency in separating and quantifying components in liquid mixtures, even in the presence of diluted solutes. On the other hand, peak overlap in chromatograms, especially among glycerol, DHA, and FA, poses a notable challenge in the analysis of GOR products. This study introduces a quantification method aimed at resolving peak overlaps in HPLC analysis of GOR products. Initially, we examine the optical properties of glycerol and GOR products to identify optimal wavelengths for spectrophotometric HPLC analysis and detection. Subsequently, we propose an algebraic approach to resolve the peak overlap of glycerol, DHA, and FA using various detectors, including the refractive index detector (RID) and the variable wavelength detector (VWD). This method is applied to analyze the GOR products of undoped, nonco-catalyzed nanoporous BiVO
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