Evidence map›Paper›PMID 40190537›Full record

ArticleAfrican health sciences2024

In-vitro scientific validation of anti-inflammatory activity of

Sharmistha Dutta, Khushbu Nishad, Talambedu Usha, Nijalingappa Ramesh, Sushil Kumar Middha

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in African health sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sharmistha DuttaDepartment, Biotechnology, School of Applied Sciences Reva University, Rukmini Knowledge Park, Bengaluru-560 064, India.
Khushbu NishadDepartment, Biotechnology, School of Applied Sciences Reva University, Rukmini Knowledge Park, Bengaluru-560 064, India.
Talambedu UshaDepartment of Biochemistry, Maharani Lakshmi Ammanni College For Women, Bengaluru-560 012, India.
Nijalingappa RameshDepartment, Biotechnology, School of Applied Sciences Reva University, Rukmini Knowledge Park, Bengaluru-560 064, India.
Sushil Kumar MiddhaDepartment of Biotechnology, Maharani Lakshmi Ammanni College For Women, Bengaluru-560 012, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: The induction of the inflammatory cascade results in the production of a number of inflammatory mediators, including prostaglandin E2 (PGE2), nitric oxide (NO), and proinflammatory cytokines like TNF-, IL-, and IL-6. This study examined the cytotoxicity and anti-inflammatory properties of a methanolic crude extract of Punica granatum L. peel (PPM) on monocytic leukaemia cell line (THP-1). Materials and methods: The PPM along with Quercetin as reference was used to assess the cytotoxic effect on THP-1 cells and describe its effect on pro-inflammatory cytokines such as COX-2, TNF-α, IL-6 against cancer cell line by flow cytometry. Results: The percentage of viable cells significantly decreased which correlates to non-toxicity whereas quercetin was found to be highly toxic, the IC50 could not be calculated because of drug precipitation. There was a significant decrease in the expressions of inflammatory cytokines upon pre-treatment of the cells with PPM prior to LPS stimulation. Conclusion: Our findings indicate that no cytotoxicity was observed after the treatment of THP-1 cells with PPM (25-400 µg/ml), but at higher concentration (400µg/ml), the cell viability decreased to 84% and attenuated the expression level of inflammatory cytokines. The inhibitory effect of the extract on pro-inflammatory factors production may provide a theoretical source on upcoming treatment of inflammation.

Indexed as

Anti-Inflammatory AgentsPlant ExtractsPomegranateCell Line, TumorCell SurvivalCyclooxygenase 2CytokinesHumansInterleukin-6MonocytesQuercetinTHP-1 CellsTumor Necrosis Factor-alphaAnti-Inflammatory AgentsCyclooxygenase 2CytokinesInterleukin-6Plant ExtractsQuercetinTumor Necrosis Factor-alphaanti-inflammationcytotoxicityMonocytic leukaemia cell linePunica granatum L

Identifiers

PMID40190537
PMCPMC11970145

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.