ArticleReproductive biology and endocrinology : RB&E2025
Ovarian tissue cryopreservation for fertility preservation: a two-decade single-center experience with 451 children and adolescents.
Article in Reproductive biology and endocrinology : RB&E, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Enhancement of angiogenesis and follicular survival using growth factors during ovarian tissue transplantation: a systematic review.Archives of gynecology and obstetrics · 2025Pooled it
- Clinical Management of Ovarian Hyperstimulation Syndrome with Furosemide at a Tertiary Referral Center-A Retrospective Data Analysis.Journal of clinical medicine · 2026Article
- Laparoscopic Surgery for Pediatric Malignant Ovarian Germ Cell Tumors After Neoadjuvant Chemotherapy: A Single-Center Retrospective Study.Journal of clinical medicine · 2026Article
- The role of anti-Müllerian hormone as a therapeutic agent to preserve the ovarian follicle pool during chemotherapy.Journal of assisted reproduction and genetics · 2026Review
- Fertility preservation in women: enhanced neovascularization, follicle viability and tissue integrity in cryopreserved human ovarian cortex by remaining medulla tissue.Journal of ovarian research · 2026Article
- Progress in research on the effects of Platelet-Rich Plasma (PRP) ovarian injection on early ovarian reserve dysfunction and premature ovarian insufficiency.Journal of ovarian research · 2026Review
- Identification of cryosensitive niches and a targetable FOS/AP‑1 program in the human ovarian cortex by single‑cell and spatial transcriptomics.BMC medicine · 2026Article
- Low-Intensity pulsed ultrasound reduces ovarian cryopreservation injury through the Notch signaling pathway.Reproductive biology and endocrinology : RB&E · 2026Article
- Ovarian tissue quality assessment and fertility preservation strategies enabled by multi-omics and artificial intelligence: current applications and clinical perspectives.Frontiers in physiology · 2026Review
- Ethical perspectives of fertility preservation through experts' opinions.Frontiers in reproductive health · 2026Article
- Geographic patterns of ovarian tissue cryopreservation utilisation across age groups in a nationwide multicentre cohort.Human reproduction open · 2026Article
- Cryopreservation of Ovarian Cortex Tissue.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Identifying ovarian cortex cell subpopulations using multicolor flow cytometry.Journal of ovarian research · 2025Article
- Three-dimensional visualisation of human ovarian follicles using a whole-mount immunolabelling and optical tissue clearing method.Reproduction & fertility · 2025Article
- Expression of growth factors in buffalo ovarian tissue across different follicular developmental stages.Archives of gynecology and obstetrics · 2025Article
- Return rates for the use of ovarian tissue cryopreserved prior to gonadotoxic treatment as fertility preservation: a systematic review.Human reproduction open · 2025Article
- Fertility preservation methods in children with the risk of female hypogonadism - possibilities and limitations.Pediatric endocrinology, diabetes, and metabolism · 2025Review
- Oncofertility in Prepubertal Girls: A Qualitative Study of Canadian and French Pediatric Oncologists' Perspectives on Ovarian Tissue Cryopreservation.The Journal of law, medicine & ethics : a journal of the American Society of Law, Medicine & Ethics · 2025Article
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5 authors.
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Abstract
backgroundOvarian tissue cryopreservation (OTC) is the only fertility preservation option for premenarcheal girls before gonadotoxic treatment, but is still considered to be experimental in pediatric patients. This study investigated storage behaviors across different age groups to refine counseling approaches for pediatric patients.
methodsThis retrospective study analyzed data from children (0-14 years), adolescents (15-19 years), and adults (≥ 20 years) who underwent OTC between 2000-2021 at the University Hospital Bonn's cryobank. Comparison to adults (age ≥ 20 years) was conducted.
resultsOf 2,475 patients, 6% were children and 12% adolescents. Sarcoma was most common in children, lymphoma in adolescents. Adults had longer active storage than children (5.5 vs. 4.7 years, p = 0.011), but for active storage ≥ 10 years, children and adolescents stored longer than adults (13.1 and 12.6 vs. 11.8 years, p ≤ 0.01). The proportion of adolescents increased, while that of children decreased in long-term storage. Median ovarian cortex surface before cryopreservation was 3.5 cm
conclusionsChildren and adolescents represent a small subset of OTC patients, with indications linked to common pediatric malignancies. For active storage ≥ 10 years, they store longer than adults, likely due to delayed reproduction or awaiting in vitro growth / in vitro maturation in hematological cases. Overall, adults store longer, but adolescent storage has risen over time possibly due to higher child mortality and previously limited OTC use in younger patients. Mean ovarian cortex surface data may guide pediatric tissue harvest recommendations, with unilateral oophorectomy advised. Fertility preservation counseling and cost coverage should be standard for pediatric patients undergoing gonadotoxic treatment. A tailored approach to OTC indications is essential, especially in high-mortality cancers like leukemia or sarcoma. Favorable pregnancy rates observed, even without OTT, suggest possible OTC overutilization, highlighting the need for individualized strategies and careful clinical decision-making to balance risks and preserve reproductive potential.
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