Evidence map›Paper›PMID 40186873›Full record

ReviewOrganogenesis2025

Calcium Imaging in Vivo: How to Correctly Select and Apply Fiber Optic Photometric Indicators.

Lanxia Wu, Wenxuan Sun, Linjie Huang, Lin Sun, Jinhua Dou, Guohua Lu

Abstract readReview
In one paragraph

Review in Organogenesis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Lanxia WuSchool of Psychology, Shandong Second Medical University, Weifang, Shandong, China.
Wenxuan SunSchool of Clinical Medicine, Shandong Second Medical University, Weifang, Shandong, China.
Linjie HuangSchool of Clinical Medicine, Shandong Second Medical University, Weifang, Shandong, China.
Lin SunSchool of Psychology, Shandong Second Medical University, Weifang, Shandong, China.
Jinhua DouMental Health Education Center, Shandong Second Medical University, Weifang, Shandong, China.
Guohua LuSchool of Psychology, Shandong Second Medical University, Weifang, Shandong, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fiber-photometric is a novel optogenetic method for recording neural activity in vivo, which allows the use of calcium indicators to observe and study the relationship between neural activity and behavior in free-ranging animals. Calcium indicators also convert changes in calcium concentration in cells or tissues into recordable fluorescent signals, which can then be observed using the system of fiber-photometric. To date, there is a paucity of relevant literature on the proper selection and application of fiber-photometric indicators. Therefore, this paper will detail how to correctly select and apply fiber-photometer indicators in four sections: the basic principle of optical fiber photometry, the selection of calcium fluorescent probes and viral vector systems, and the measurement of specific expression of fluorescent proteins in specific tissues. Therefore, the correct use of suitable fiber optic recording indicators will greatly assist researchers in exploring the link between neuronal activity and neuropsychiatric disorders.

Indexed as

CalciumFiber Optic TechnologyOptical FibersPhotometryAnimalsFluorescent DyesHumansOptogeneticsCalciumFluorescent DyesCalcium imagingfibre photometrygenetically encoded calcium indicators(GECIs)virus selection

Identifiers

PMID40186873
PMCPMC11980459

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.