β-(1 → 3) Linked Sulfated Polygalactan from a Seaweed-associated Bacillus velezensis MTCC 13097: A Potential Lead Against Human Hepatocellular Adenocarcinoma.

Sumayya Asharaf, Kajal Chakraborty, Silpa Kunnappilly Paulose, Chesvin Varghese, Shubhajit Dhara

Abstract read

In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Sumayya Asharaf *Marine Biotechnology Fish Nutrition and Health Division, ICAR-Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, 682018, Kerala State, India.

Kajal Chakraborty *Marine Biotechnology Fish Nutrition and Health Division, ICAR-Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, 682018, Kerala State, India. kajal_cmfri@yahoo.com.

Silpa Kunnappilly PauloseMarine Biotechnology Fish Nutrition and Health Division, ICAR-Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, 682018, Kerala State, India.

Chesvin VargheseMarine Biotechnology Fish Nutrition and Health Division, ICAR-Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, 682018, Kerala State, India.

Shubhajit DharaMarine Biotechnology Fish Nutrition and Health Division, ICAR-Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, 682018, Kerala State, India.

Funding

Indian Council of Agricultural Research MBT/HM/SUB/23

6 · The paper itself

Abstract

Among tumors, liver cancer has an inferior prognosis. Therefore, exploring alternative strategies to improve the effectiveness of treatment for this ailment is of utmost urgency. In this study, we focused on analyzing the anti-cancer properties of bacterial exopolysaccharide from Bacillus velezensis associated with the seaweed Sargassum wightii against hepatocellular adenocarcinoma. A culture-dependent method was used to isolate heterotrophic B. velezensis, which was then evaluated for its antioxidant and anti-cancer properties. A β-(1 → 3) linked sulfated polygalactan exopolysaccharide (BVEP-2) was isolated from the bacterial extract and characterized by spectroscopic analysis. The anti-cancer property was analyzed through assays involving 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), neutral red uptake (NRU), and apoptosis (by annexin V-FITC/PI staining) against the human hepatocellular adenocarcinoma cell line (HepG2). BVEP-2 demonstrated potential cytotoxicity in the MTT assay (IC

Indexed as

Antineoplastic AgentsBacillusCarcinoma, HepatocellularLiver NeoplasmsPolysaccharides, BacterialSargassumSeaweedAntioxidantsApoptosisGalactansHep G2 CellsHumansAntineoplastic AgentsAntioxidantsGalactansPolysaccharides, BacterialAnti-cancer agentBacillus velezensis MTCC13097Heterotrophic bacteriumHuman hepatocellular adenocarcinomaβ-(1 → 3) linked sulfated polygalactan

Identifiers

PMID40186775

DOI10.1007/s10126-025-10447-8

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.