Evidence map›Paper›PMID 40186234›Full record

ArticleMicrobial cell factories2025

Determining metabolic mechanism linking phospholipids and docosahexaenoic acid through phosphatidylcholine synthesis by phosphocholine cytidylyltransferase (CCT) overexpression in Schizochytrium sp.

Xiaowen Cui, Tingting Chen, Yizhen Meng, Xueshan Pan, Ruizhe Wu, Yinghua Lu, Chuanyi Yao, Xihuang Lin, Xueping Ling

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Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Xiaowen CuiDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, P.R. China.
Tingting ChenDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, P.R. China.
Yizhen MengDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, P.R. China.
Xueshan PanDepartment of Biochemistry and Molecular Biology, School of Laboratory Medicine, Bengbu Medical University, Bengbu, P.R. China.
Ruizhe WuDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, P.R. China.
Yinghua LuDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, P.R. China. ylu@xmu.edu.cn.
Chuanyi YaoDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, P.R. China.
Xihuang LinAnalysis and Test Center, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, P. R. China.
Xueping LingDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, P.R. China. xpling@xmu.edu.cn.

Funding

National Natural Science Foundation of China 22478324Natural Science Foundation of Xiamen Municipality 3502Z20227183
6 · The paper itself

Abstract

The polyunsaturated fatty acid (PUFA) metabolism of Schizochytrium, an excellent oil-producing microorganism, is closely related to phosphatidylcholine (PC) synthesis, which favors the migration and accumulation of docosahexaenoic acid (DHA). Phosphocholine cytidylyltransferase (CCT), a key enzyme involved in PC synthesis, profoundly impacts lipid metabolism in plants; however, few studies have focused on CCT in microorganisms. We investigated the effects of CCT overexpression on lipid metabolism in Schizochytrium sp. CCT overexpression slightly inhibited cell growth, but significantly promoted total lipid synthesis. Compared to the wild-type strain, PUFA content and DHA production in the CCT-overexpressing strain (SR21-CCT) increased by about 49% and 46%, respectively. Analysis of phospholipids and quantitative real-time PCR revealed that CCT overexpression enhanced phospholipid synthesis, especially by strengthening glycerophosphorylcholine acylation and de novo PC synthesis pathways, which promote DHA esterification to PC and DHA accumulation in triacylglycerols. This study helps decipher the mechanism correlating phospholipid metabolism and DHA production.

Indexed as

Choline-Phosphate CytidylyltransferaseDocosahexaenoic AcidsPhosphatidylcholinesPhospholipidsStramenopilesLipid MetabolismCholine-Phosphate CytidylyltransferaseDocosahexaenoic AcidsPhosphatidylcholinesPhospholipidsDocosahexaenoic acidPhosphatidylcholine pathwayPhosphocholine cytidylyltransferasePhospholipid synthesis

Identifiers

PMID40186234
PMCPMC11969949

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