In one paragraphArticle in Journal of veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
5 · Who and what moneyAuthors and funding
8 authors.
Shucheng ZongBeijing Key Laboratory of Traditional Chinese Veterinary Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China.ORCID https://orcid.org/0009-0003-3847-2986 Xinze LiuKey Laboratory of Veterinary Biological Products and Chemical Drugs, Ministry of Agriculture and Rural Affairs, Engineering and Technology Research Center for Beijing Veterinary Peptide Vaccine Design and Preparation, Zhongmu Institutes of China Animal Husbandry Industry Co. Ltd, Beijing 100095, China.ORCID https://orcid.org/0009-0004-0442-5572 Wen PanKey Laboratory of Veterinary Biological Products and Chemical Drugs, Ministry of Agriculture and Rural Affairs, Engineering and Technology Research Center for Beijing Veterinary Peptide Vaccine Design and Preparation, Zhongmu Institutes of China Animal Husbandry Industry Co. Ltd, Beijing 100095, China.ORCID https://orcid.org/0009-0000-1847-9724 Fucheng GuoKey Laboratory of Veterinary Biological Products and Chemical Drugs, Ministry of Agriculture and Rural Affairs, Engineering and Technology Research Center for Beijing Veterinary Peptide Vaccine Design and Preparation, Zhongmu Institutes of China Animal Husbandry Industry Co. Ltd, Beijing 100095, China.ORCID https://orcid.org/0009-0004-7323-3750 Jinyu FuKey Laboratory of Veterinary Biological Products and Chemical Drugs, Ministry of Agriculture and Rural Affairs, Engineering and Technology Research Center for Beijing Veterinary Peptide Vaccine Design and Preparation, Zhongmu Institutes of China Animal Husbandry Industry Co. Ltd, Beijing 100095, China.ORCID https://orcid.org/0009-0007-8308-5730 Zhongyin LiuBeijing Key Laboratory of Traditional Chinese Veterinary Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China.ORCID https://orcid.org/0009-0002-3119-0946 Jin XiaoKey Laboratory of Veterinary Biological Products and Chemical Drugs, Ministry of Agriculture and Rural Affairs, Engineering and Technology Research Center for Beijing Veterinary Peptide Vaccine Design and Preparation, Zhongmu Institutes of China Animal Husbandry Industry Co. Ltd, Beijing 100095, China. xjpeptide@126.com.ORCID https://orcid.org/0009-0008-6652-0208 Shengkui XuBeijing Key Laboratory of Traditional Chinese Veterinary Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China. skxu0721@163.com.ORCID https://orcid.org/0009-0003-4698-8392 Funding
Beijing Municipal Education Commission KM202310020004Beijing University of Agriculture QJKC2022030National Natural Science Foundation of China 32202825
6 · The paper itselfAbstract
importanceThe Japanese encephalitis virus (JEV) is a massive threat to the pig-raising industry and attenuated-live vaccines are used widely to prevent JEV infections. Primary hamster kidney cells and Vero cells are mainly used to propagate JEV-attenuated vaccines.
objectiveThis study aimed to construct porcine mesangial cells (PMCs) for JEV cultivation and study its characteristics.
methodsAn immunofluorescence assay was used to analyze the homogeneity of PMCs. Karyotypic analysis and flow cytometry were used to detect the cell chromosome numbers and cell cycle, respectively. TCID₅₀ was used to measure the viral titers.
resultsThe prepared PMCs were uniform in shape and without variation in chromosome numbers. The PMCs exhibit strong division and proliferation ability, and the JEV could replicate efficiently, having a similar cytopathic effect to that in Vero cells. The viral titer results showed that the JEV grows faster in PMCs than in Vero cells. CONCLUSIONS AND RELEVANCE: This study generated an immortalized PMC cell line suitable for cultivating the JEV, which has significance for producing pig JEV-attenuated vaccines.
Indexed as
Encephalitis Virus, JapaneseMesangial CellsVirus CultivationAnimalsCell LineCell Line, TransformedChlorocebus aethiopsSwineVero Cellscell lineJapanese encephalitis virusvaccineviral titervirus cultivation
Identifiers
PMID40183907
PMCPMC11972940
What OpenQuestion holds
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LicenceCC BY-NC
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