Evidence map›Paper›PMID 40183544›Full record

ArticleJournal of bacteriology2025

Rapid screening and identification of genes involved in bacterial extracellular membrane vesicle production using a curvature-sensing peptide.

Hiromu Inoue, Kenichi Kawano, Jun Kawamoto, Takuya Ogawa, Tatsuo Kurihara

Abstract read
In one paragraph

Article in Journal of bacteriology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Hiromu InoueInstitute for Chemical Research, Kyoto University, Uji, Kyoto, Japan.ORCID 0009-0007-9778-4786
Kenichi KawanoGraduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
Jun KawamotoInstitute for Chemical Research, Kyoto University, Uji, Kyoto, Japan.ORCID 0000-0002-5025-5613
Takuya OgawaInstitute for Chemical Research, Kyoto University, Uji, Kyoto, Japan.ORCID 0000-0002-0714-482X
Tatsuo KuriharaInstitute for Chemical Research, Kyoto University, Uji, Kyoto, Japan.ORCID 0000-0002-7777-1378

Funding

Institute for Chemical Research, Kyoto UniversityInstitute for Fermentation, Osaka L-2019-2-012Japan Society for the Promotion of Science JP18K19178Japan Society for the Promotion of Science JP20K05786Japan Society for the Promotion of Science JP20K20570Japan Society for the Promotion of Science JP23H02127
6 · The paper itself

Abstract

Bacteria secrete extracellular membrane vesicles (EMVs). Physiological functions and biotechnological applications of these lipid nanoparticles have been attracting significant attention. However, the details of the molecular basis of EMV biogenesis have not yet been fully elucidated. In our previous work, an N-terminus-substituted FAAV peptide labeled with nitrobenzoxadiazole (NBD; nFAAV5-NBD) was developed. This peptide can sense the curvature of a lipid bilayer and selectively bind to EMVs even in the presence of cells. Here, we applied nFAAV5-NBD to a genome-wide screening of hyper- and hypo-vesiculation transposon mutants of a Gram-negative bacterium,

Indexed as

Bacterial ProteinsExtracellular VesiclesPeptidesShewanellaDNA Transposable ElementsGene Expression Regulation, BacterialBacterial ProteinsDNA Transposable ElementsPeptidescurvature-sensing peptideextracellular membrane vesicleShewanella vesiculosavesiculation

Identifiers

PMID40183544
PMCPMC12096838

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.