ArticleBrain and behavior2025
Multiplex Immunofluorescent Batch Labeling of Marmoset Brain Sections.
Article in Brain and behavior, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Improved Post-Processing Framework for Whole Slide Imaging and 3D Reconstruction.Microscopy research and technique · 2026Article
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9 authors.
Funding
Abstract
purposeThe common marmoset is a small nonhuman primate that has emerged as a valuable animal model in neuroscience research. Accurate analysis of brain tissue is crucial to understand marmoset neurophysiology and to model neurodegenerative diseases. Many studies to date have complemented magnetic resonance imaging (MRI) with histochemical staining rather than immunofluorescent labeling, which can generate more informative and higher resolution images. There is a need for high-throughput immunolabeling and imaging methodologies to generate resources for the burgeoning marmoset field, particularly brain histology atlases to display the organization of different cell types and other structures. METHODS AND
findingsHere, we have characterized a set of marmoset-compatible fluorescent dyes and antibodies that label myelin, axons, dendrites, and the iron-storage protein ferritin, and developed a batch-style multiplex immunohistochemistry protocol to uniformly process large numbers of tissue slides for multiple cell-type specific markers.
conclusionWe provide a practical guide for researchers interested in harnessing the potential of marmoset models to advance understanding of brain structure, function, and pathophysiology.
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