ArticleScientific reports2025
Improved production of thermostable pectinase from novel Bacillus siamensis (BIOSMNF45) using computational-conventional approach.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Eco-applicable pectinase from Bacillus infantis (SA2): enzyme production, optimization, protein profiling, and pesticide degradation approaches using molecular docking.Archives of microbiology · 2026Article
- Alkaline‑adapted pectinase from polyextremotolerant Bacillus amyloliquefaciens via agro-wastes valorization: dual biocatalyst and multifunctional bioinoculant.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The demand for thermostable pectinase in the global commercial market has expanded progressively at a rapid pace. Many industrial processes are operated at elevated temperatures to break down plant polysaccharides (pectin) owing to the significant use of thermotolerant pectinase. Therefore, the study aimed to optimize the medium constituents for enhanced pectinase production from a thermotolerant pectinolytic bacteria (BIOSMNF45) in combination with the one-variable-at-a-time (OVAT) and response surface methodology (RSM). The isolated BIOSMNF45 with high thermostable pectinase activity was identified as Bacillus siamensis by 16S rRNA sequencing. OVAT technique was used in the preliminary screening of different variables (incubation time, incubation temperature, pH, carbon%, organic nitrogen%, and inorganic nitrogen%) before proceeding to the RSM study. The OVAT result showed that the highest production of thermostable pectinase was attained at 1.00% (w/v) carbon (pectin), 0.40% organic nitrogen (yeast extract), pH 6.00, temperature 45℃ and an agitation of 150 rpm for 24 h incubation. Furthermore, the RSM outcomes revealed that the optimized fermentation conditions boost the thermostable pectinase yield from Bacillus siamensis (BIOSMNF45) by 6.7 folds. It also suggested that the percentage of carbon and temperature showed the highest impact on improved pectinase production compared to other factors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.