ArticleInternational journal of implant dentistry2025
Aggregatibacter actinomycetemcomitans induces biofilm formation of Streptococcus sanguinis on titanium implants.
Article in International journal of implant dentistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
purposeThis study aims to investigate the distinct behaviors of single-species and dual-species biofilms formed by Streptococcus sanguinis and Aggregatibacter actinomycetemcomitans on different titanium and implant surfaces. Four types of surfaces were examined: two clinically used implant surfaces, a super-polished surface and a sand-blasted surface of grade 4 titanium.
methodsSpecimens were incubated with single- and dual-species biofilms for 24 h. Biofilm formation was determined based on the amount of total DNA extracted from the bacteria. In order to specifically determine the biofilm formation of Streptococcus sanguinis, qPCR experiments were carried out. Staining followed by fluorescence microscopy was employed to verify the efficiency of the washing steps.
resultsBiofilm formation by single- and dual-species cultures was observed on all tested implant surfaces. However, a clear influence of surface characteristics on biofilm formation could not be conclusively demonstrated. Mixed cultures of S. sanguinis and A. actinomycetemcomitans (AAC) exhibited increased biofilm formation through the enhanced DNA amount of S. sanguinis. In contrast, this effect was not observed in dual-species cultures of Staphylococcus epidermidis and S. sanguinis.
conclusionAAC promotes biofilm formation of S. sanguinis, highlighting the significant role of AAC in enhancing biofilm development. Conversely, a definitive conclusion regarding the correlation between titanium implant surface roughness and biofilm formation was not possible. However, our results suggest a tendency that dual-species biofilm formation may be influenced by surface structure.
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