Evidence map›Paper›PMID 40177670›Full record

ArticleFrontiers in veterinary science2025

Mesenchymal stromal cell isolation from pond slider (

Valentina Andreoli, Alessandro Vetere, Virna Conti, Martina Gavezzoli, Priscilla Berni, Roberto Ramoni, Giuseppina Basini, Giordano Nardini, Igor Pelizzone, Stefano Grolli and 1 more

Abstract read
In one paragraph

Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Valentina Andreoli *Department of Veterinary Medical Science, University of Parma, Parma, Italy.
Alessandro Vetere *Department of Veterinary Medical Science, University of Parma, Parma, Italy.
Virna ContiDepartment of Veterinary Medical Science, University of Parma, Parma, Italy.
Martina GavezzoliDepartment of Veterinary Medical Science, University of Parma, Parma, Italy.
Priscilla BerniDepartment of Veterinary Medical Science, University of Parma, Parma, Italy.
Roberto RamoniDepartment of Veterinary Medical Science, University of Parma, Parma, Italy.
Giuseppina BasiniDepartment of Veterinary Medical Science, University of Parma, Parma, Italy.
Giordano NardiniClinica Veterinaria Modena Sud, Modena, Italy.
Igor PelizzoneDepartment of Veterinary Medical Science, University of Parma, Parma, Italy.
Stefano GrolliDepartment of Veterinary Medical Science, University of Parma, Parma, Italy.
Francesco Di IanniDepartment of Veterinary Medical Science, University of Parma, Parma, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Mesenchymal stromal cells (MSCs) hold great clinical potential in veterinary regenerative medicine. However, a notable gap exists in the literature regarding the isolation and characterization of these cells in reptiles. The objective of this study was to evaluate the feasibility of isolating adipose tissue-derived mesenchymal stem cells (MSCs) from pond slider ( Methods: Adipose tissue samples were obtained from five animals and processed using an enzymatic procedure. The resulting cell suspension was subsequently cultured at 28°C in a controlled atmosphere with 5% CO Results: The cells demonstrated the capacity to differentiate into adipogenic, chondrogenic, and osteogenic lineages. RT-PCR revealed the expression of CD105, CD73, CD44, and CD90, whereas CD34 and HLA-DRA were not expressed. Sequence homology analysis demonstrated that the amplicons matched the sequences reported in the Discussion: The results demonstrate the feasibility of isolating MSC-like cells from chelonian adipose tissue and underscore their potential for application in regenerative medicine for both companion reptiles and endangered wild species.

Indexed as

cheloniamesenchymal stromal cellsregenerative medicinereptile medicinetortoiseturtlesveterinary regenerative medicine

Identifiers

PMID40177670
PMCPMC11963382

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.