Background: Human immunodeficiency virus (HIV) is still a major global public health problem. Sub-Saharan Africa remains the most severely affected, accounting for 69% of the people living with HIV worldwide. Currently, Pre-Exposure Prophylaxis [PrEP] pills are offered but are highly affected by non-adherence. Long-acting injectable PrEP has the potential to improve adherence. Objective: This study aimed to explore awareness and willingness to use long-acting injectable HIV pre-exposure prophylaxis among users and non-PrEP user female barmaids in Ubungo municipality in Dar es Salaam, Tanzania. Methods: This phenomenological study used in-depth interviews to collect data. A total of 17 study participants were purposively selected. Audio-recorded interviews were transcribed verbatim and translated into English. A thematic approach was used to identify patterns in the data. Key themes were coded using NVivo14 and then summarized into key findings. Findings: The findings revealed that participants who were PrEP pill users and non-users were aware of PrEP pills. Few of the PrEP pill users were aware of the long-acting injectable PrEP. The majority of both users and non-users of PrEP pills were willing to use the long-acting injectable PrEP. A few PrEP non-users expressed fear for PrEP, citing safety, and insisted on continuous condom use. Conclusion: The majority of participants are aware of and willing to use long-acting injectable PrEP when made available. The initiation of HIV long-acting injectable PrEP has the potential to increase protection options among female barmaids who are a population at risk of HIV infection.