Method for Imaging and Quantifying Molecular Tensions at Cell-Cell Junctions Using DNA-Based Fluorescent Probes.

Priyanka Bhattacharyya, Qian Tian, Mingxu You

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

SynNotch Receptors for Visualizing Immunoreceptor Force Transmission and Downstream Signaling In Vivo.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Priyanka Bhattacharyya *Department of Chemistry, University of Massachusetts, Amherst, MA, USA.

Qian Tian *Department of Chemistry, University of Massachusetts, Amherst, MA, USA.

Mingxu YouDepartment of Chemistry, University of Massachusetts, Amherst, MA, USA. mingxuyou@umass.edu.

Funding

Defining Mechanical Landscapes at Cell-Cell JunctionsR35GM133507 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI Mingxu You · 2019 to 2026

$2.8M

NIGMS NIH HHS R35 GM133507

6 · The paper itself

Abstract

Mechanical forces play crucial roles in regulating cellular communications. It has been challenging to measure intercellular forces due to the lack of proper tools. We have recently developed lipid-modified DNA probes that can anchor on the cell membranes and detect intercellular tensions through targeting particular cell surface receptors of interest. Herein, we describe the methods to synthesize, characterize, and apply these DNA-based fluorescent probes for imaging and quantifying intercellular forces.

Indexed as

DNADNA ProbesFluorescent DyesIntercellular JunctionsMolecular ImagingAnimalsCell CommunicationCell MembraneHumansMicroscopy, FluorescenceDNADNA ProbesFluorescent DyesCell membrane analysisDNA probesFluorescence microscopyIntercellular forcesLipid-DNA conjugate

Identifiers

PMID40175872

DOI10.1007/978-1-0716-4394-5_10

PMCPMC12599496

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.