Evidence map›Paper›PMID 40175864›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Rapid and High-Yielding Purification of DNA Self-Assembled Structures by Aqueous Two-Phase System.

Marcos K Masukawa, Masahiro Takinoue

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Marcos K MasukawaLife-Like Materials and Systems, Department of Chemistry, University of Mainz, Mainz, Germany. marcos.masukawa@uni-mainz.de.
Masahiro TakinoueDepartment of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aqueous two-phase systems (ATPS) of dextran and polyethylene glycol (PEG) enable the purification of DNA structures such as DNA origami and DNA nanotubes in times as short as 10 min. This method, which has recovery yields >90% for a typical DNA origami, owes its efficiency to the highly selective partition of the DNA structures in the dextran phase of these emulsions. This purification method is carried out in conditions that promote the structural stability of these structures, making it particularly suitable for DNA nanotechnology. In this protocol, we will describe the materials and methods for purifying DNA origami and quantifying the purification yield by agarose electrophoresis and image analysis.

Indexed as

DNAWaterDextransElectrophoresis, Agar GelEmulsionsNanotechnologyNucleic Acid ConformationPolyethylene GlycolsDextransDNAEmulsionsPolyethylene GlycolsWaterAgarose electrophoresisAqueous two-phase system (ATPS)DNA nanostructuresDNA nanotechnologyDNA nanotubesDNA origami purificationImage analysis

Identifiers

PMID40175864

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.