ArticleMethods in molecular biology (Clifton, N.J.)2025
Complex Spheroids as an Alternative: In Vivo-Like 3D Model for Investigating the Impact of Stromal Cells on the Radiation Response of Tumors.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Microfluidic Cytotongue system enables early, label-free detection of CAR-T-induced mechanical softening in tumor spheroids.Microsystems & nanoengineering · 2026Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three-dimensional environments that mimic in vivo microenvironments promote native epithelial cell polarity and differentiation by enabling cell-matrix interactions. When tumor cells were embedded as spherical cell aggregates (spheroids) in such microenvironments, particularly in semi-solid extracellular matrices, further the intimate cell-cell adhesion architecture as well as direct cell-matrix interactions can be efficiently recapitulated, including the presence of nutritional and metabolic gradients. The complexity of these spheroids can be further increased by the use of intercalating stromal cells. Here, we describe a simple and rapid method in which tumor and stromal cells are placed in hanging drop culture to generate homogenous and complex spheroids before embedding them in laminin-/collagen IV-rich basement membrane extracts (Matrigel), which in turn can be used as model system for cancer progression and to evaluate the efficacy of anticancer drugs, especially after radiation treatment.
Indexed as
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40172832What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.