ReviewApplied microscopy2025
Specimen preparation for X-ray micro-computed tomography of forest pests.
Review in Applied microscopy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Correlative x-ray microscopy and transmission electron microscopy for ultrastructural analysis of zebrafish tissue.Applied microscopy · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
X-ray micro-computed tomography (XCT) is an X-ray-based three-dimensional (3D) imaging technique that enables non-destructive imaging of both external and internal structures. It is widely used for studying biological specimens such as animals and plants. In this review, we discuss various specimen preparation methods for the technique, particularly focusing on forest pests, with six representative cases. Specimen preparation methods for forest pests can be broadly categorized into three groups based on mounting types: (i) simple mounting, (ii) liquid-cell mounting, and (iii) dry-cell mounting. The simple mounting method is particularly suitable for adult beetles due to their exoskeleton. The dehydration process minimizes specimen movement during scanning, ensuring better imaging quality. In the case of liquid-cell mounting, the specimen is immersed in a liquid medium for scanning, which effectively preserves the soft tissues of larvae and pupae. The dry-cell mounting does not involve fixation or dehydration and is particularly useful for analyzing immobilized specimens. To enhance the quality of 3D images, selecting an appropriate preparation method is essential. Since forest pests display varying sizes and types, the choice of preparation method should be based on the specific characteristics of the specimens of interest and research objectives. This review provides valuable insights for researchers and practitioners seeking to identify the most suitable and effective mounting method for XCT scanning of forest pests.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.