Evidence map›Paper›PMID 40171972›Full record

ArticleVeterinary medicine and science2025

Frequency and Sequence Analysis of Echinococcus granulosus Sensu Lato Isolated From Buffaloes in Northern Iran.

Fariborz Ghanbari, Keyhan Ashrafi, Majid Fasihi Harandi, Somayeh Abbaszadeh, Asieh Ashouri, Elham Hajialilo, Meysam Sharifdini

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Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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3 · Its place in the literature

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1 citing paper in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Fariborz GhanbariDepartment of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.ORCID 0009-0007-9194-9412
Keyhan AshrafiDepartment of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.ORCID 0000-0002-1742-1245
Majid Fasihi HarandiResearch Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.ORCID 0000-0003-3257-5389
Somayeh AbbaszadehDepartment of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.ORCID 0000-0002-0032-1635
Asieh AshouriResearch Center of Health and Environment, School of Health, Guilan University of Medical Sciences, Rasht, Iran.ORCID 0000-0002-3791-8451
Elham HajialiloDepartment of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.ORCID 0000-0003-2159-4066
Meysam SharifdiniDepartment of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.ORCID 0000-0003-2686-7748

Funding

Guilan University of Medical Sciences
6 · The paper itself

Abstract

backgroundEchinococcus granulosus sensu lato is one of the most important zoonotic parasites in many regions of the world. Cystic echinococcosis (CE) is a major economic and public health challenge in Iran.

objectiveThis study aimed to determine the prevalence and genotypes of Echinococcus granulosus isolates from slaughtered buffaloes in Guilan Province, northern Iran.

methodsWe examined 384 buffaloes at the Talesh county abattoir, isolating 42 hydatid cysts. We sequenced the mitochondrial cytochrome c oxidase subunit 1 (CO1) and NADH dehydrogenase subunit 1 (ND1) genes for genotype analysis. Molecular phylogeny and haplotype network analysis was performed for the isolates.

resultsOur results showed that 10.9% of the buffaloes were infected with hydatid cysts, with 90.5% of infections in the liver and 9.5% in the lungs. Only one liver cyst was fertile; the rest were non-fertile. Amongst the infected animals, 21.4% (9/42) were male, and 78.6% (33/42) were female. No significant gender-related difference in infection rates was observed (p = 0.057); however, a significant relationship was found between age groups and infection (p = 0.030). Sequence analysis identified all isolates as belonging to the G1 genotype of E. granulosus, with five haplotypes being identified.

conclusionThe study highlights the high prevalence of hydatid cysts in buffaloes, though fertility was low, suggesting a minor role for buffaloes in CE epidemiology in this region. Similar to other studies, our research shows that infection rates increase significantly with age.

Indexed as

BuffaloesEchinococcosisEchinococcus granulosusAnimalsFemaleGenotypeHaplotypesIranMaleNADH DehydrogenasePhylogenyPrevalenceNADH Dehydrogenasebuffalocystic echinococcosisgenotypingIran

Identifiers

PMID40171972
PMCPMC11962749

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