Evidence map›Paper›PMID 40171225›Full record

ArticleEvolutionary bioinformatics online2025

Structural and Functional Characterization of a Putative Type VI Secretion System Protein in

Nurun Nahar Akter, Md Moin Uddin, Nesar Uddin, Israt Jahan Asha, Md Soyeb Uddin, Md Arju Hossain, Fahadul Alam, Siratul Kubra Shifat, Md Abu Zihad, Md Habibur Rahman

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Article in Evolutionary bioinformatics online, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nurun Nahar AkterDepartment of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, Bangladesh.ORCID https://orcid.org/0009-0003-4730-3615
Md Moin UddinDepartment of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0009-0005-4282-2330
Nesar UddinDepartment of Apparel Engineering, Textile Engineering College, Noakhali, Bangladesh.
Israt Jahan AshaDepartment of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, Bangladesh.ORCID https://orcid.org/0009-0009-1901-3139
Md Soyeb UddinDepartment of Botany, Cox's Bazar Government College, Cox's Bazar, Bangladesh.
Md Arju HossainDepartment of Biochemistry and Biotechnology, Khwaja Yunus Ali University, Sirajganj, Bangladesh.
Fahadul AlamDepartment of Biological Sciences, Texas Tech University, TX, USA.
Siratul Kubra ShifatDepartment of Bioprocess and Genetic Engineering, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh.
Md Abu ZihadDepartment of Microbiology, Primeasia University, Dhaka, Bangladesh.
Md Habibur RahmanDepartment of Computer Science and Engineering, Islamic University, Kushtia, Bangladesh.ORCID https://orcid.org/0000-0002-5068-2690

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Objectives: The aim of this investigation is to identify and annotate the structural and functional properties of a hypothetical protein (HP) from Methods: Multiple bioinformatic tools were used to identify the homologous protein and to construct and validate its 3D structure. A 3D model was generated using SWISS-MODEL and validated using tools, developing a reliable 3D structure. The STRING and CASTp servers provided information on protein-protein interactions and active sites, identifying functional partners. Results: The putative protein was soluble, stable, and localized in the cytoplasmic membranes, indicating its biological activity. Functional annotation identified TagJ (HsiE1) within the protein, a member of the ImpE superfamily involved in the transport of toxins and a part of the bacterial type VI secretion system (T6SS). The 3-dimensional structure of this protein was validated through molecular docking involving 6 different compounds. Among these, ceforanide demonstrated the strongest binding scores, -7.5 kcal/mol for the hypothetical protein and -7.2 kcal/mol for its main template protein (PDB ID: 4UQX.1). Conclusion: Comparative genomics study suggests that the protein found in

Indexed as

Cronobacter sakazakiihypothetical proteinin-silicoSWISS-MODELT6SS

Identifiers

PMID40171225
PMCPMC11960190

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.