ReviewBiophysical journal2025
Single-molecule measurements of double-stranded DNA condensation.
Review in Biophysical journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Materials and measurement in mechanobiology.Biophysical journal · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electrostatically driven double-stranded DNA (dsDNA) condensation is critical in regulating many biological processes, including bacteriophage and virus replication and the packaging of chromosomal DNA in sperm heads. Here, we review single-molecule measurements of dsDNA condensed by cationic proteins, polypeptides, and small multivalent cations. Optical tweezers (OT) measurements of dsDNA collapsed by cationic condensing agents reveal a critical condensing force unique to each condensing agent that is tunable with condensing agent concentration and ionic strength. DNA globules visualized via atomic force microscopy, transmission electron microscopy, and cryoelectron microscopy reveal condensed dsDNA adopting several conformations including highly ordered toroids with a measurable central hole and, more recently, the maximally dense, yarn-ball-like structures observed with dsDNA condensed by the HIV-1 nucleocapsid protein. The combination of these approaches provides multifaceted insight into the shape and size of electrostatically condensed dsDNA globules and the kinetics of their formation and dissolution. We also review the physics of dsDNA condensation, including recent studies that show dsDNA globule size is tunable with ionic strength. Overall, this review provides important insights into understanding dsDNA condensate-regulated biological processes, as well as potential uses for gene delivery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.