ArticleBMC veterinary research2025
Rapid visual detection of bovine viral diarrhea virus (BVDV) using recombinase polymerase amplification with SYBR green I.
Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- A miniaturized, frugal RPA assay for genus-level detection of Paracoccidioides spp. in resource-limited endemic settings.PLoS neglected tropical diseases · 2026Article
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8 authors.
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Abstract
backgroundBovine diarrhea virus (BVDV) is considered to be the most common pathogen causing severe diarrhea in cattle worldwide and can cause Bovine viral diarrhea (BVD). Clinical manifestations of fever, diarrhea, ulcers, and abortions, resulting in significant economic losses to the cattle industry. The development of an efficient, rapid and sensitive assay suitable for field conditions is of great significance for its early detection. Recombinase polymerase amplification (RPA) is a novel nucleic acid amplification method that has been widely used in the diagnosis of infectious diseases.
resultsWe developed a rapid assay (RPAS) combining RPA with SYBR Green I for the detection of BVDV. The BVDV RPAS assay was performed at 37 °C in 25 min. The minimum detection limit of the RPAS assay is 1 × 10
conclusionsThe BVDV RPAS assay established in this study has high sensitivity and specificity, and is expected to be a powerful tool for the prevention and control of BVD.
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