Evidence map›Paper›PMID 40168997›Full record

ArticleCell host & microbe2025

Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.

Emily G Armbruster, Phoolwanti Rani, Jina Lee, Niklas Klusch, Joshua Hutchings, Lizbeth Y Hoffman, Hannah Buschkaemper, Eray Enustun, Benjamin A Adler, Koe Inlow and 17 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell host & microbe, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. The biology of jumbo phages.Nature communications · 2026
    Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Bacteriophage genome-wide transposon mutagenesis.bioRxiv : the preprint server for biology · 2025
    Article
  10. Review
  11. Article
  12. The Biology of Nucleus-Forming Jumbo Phages.Annual review of genetics · 2025
    Review
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors.

Emily G ArmbrusterSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Phoolwanti RaniSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA; Howard Hughes Medical Institute, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Jina LeeSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Niklas KluschSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA; Howard Hughes Medical Institute, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Joshua HutchingsSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA; Howard Hughes Medical Institute, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Lizbeth Y HoffmanSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA; Howard Hughes Medical Institute, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Hannah BuschkaemperSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA; Gene Center and Department of Biochemistry, Ludwig Maximilian University of Munich, 80539 Munich, Germany.
Eray EnustunSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Benjamin A AdlerCalifornia Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
Koe InlowSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Arica R VanderWalSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA; Howard Hughes Medical Institute, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Madelynn Y HoffmanSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Daksh DakshNational Institute of Science, Education and Research (NISER), Bhubaneshwar 752050, Orissa, India.
Ann AindowSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Amar DeepDepartment of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Zaida K RodriguezSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Chase J MorganSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Majid GhassemianBiomolecular and Proteomics Mass Spectrometry Facility, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Thomas G LaughlinSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Emeric CharlesInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Brady F CressInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
David F SavageInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
Jennifer A DoudnaCalifornia Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; MBIB Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Kit PoglianoSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Kevin D CorbettSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA; Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, San Diego, CA 92093, USA.
Elizabeth VillaSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA; Howard Hughes Medical Institute, University of California, San Diego, La Jolla, San Diego, CA 92093, USA. Electronic address: evilla@ucsd.edu.
Joe PoglianoSchool of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA. Electronic address: jpogliano@ucsd.edu.

Funding

Pathways in Biological Sciences Training ProgramT32GM133351 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Matthew Daugherty, Randolph Y. Hampton · 2020 to 2026
$9.9M
U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
FORESPORE ENGULFMENT DURING B SUBTILIS SPORULATIONR01GM057045 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI POGLIANO, KIT J · 1998 to 2021
$6.1M
Molecular and cellular biology of the phage nucleus and spindleR01GM129245 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI POGLIANO, JOSEPH A · 2018 to 2025
$4.2M
Molecular mechanisms of nucleic acid recognition and maintenance in meiosis and innate immunityR35GM144121 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Kevin Daniel Corbett · 2022 to 2026
$2.7M
Elyra 7 Microscope with Lattice SIM2S10OD030505 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ZHENG, BINHAI · 2022 to 2022
$600k
Investigating the mechanism of infection and therapeutic potential of Pseudomonas nucleus-forming phagesF30AI176696 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Chase Morgan · 2024 to 2026
$128k
NIAID NIH HHS F30 AI176696NIGMS NIH HHS R01 GM057045NIGMS NIH HHS R01 GM129245NIGMS NIH HHS R35 GM144121NIGMS NIH HHS T32 GM133351NIH HHS S10 OD030505NINDS NIH HHS P30 NS047101
6 · The paper itself

Abstract

Many eukaryotic viruses require membrane-bound compartments for replication, but no such organelles are known to be formed by prokaryotic viruses. Bacteriophages of the Chimalliviridae family sequester their genomes within a phage-generated organelle, the phage nucleus, which is enclosed by a lattice of the viral protein ChmA. We show that inhibiting phage nucleus formation arrests infections at an early stage in which the injected phage genome is enclosed within a membrane-bound early phage infection (EPI) vesicle. Early phage genes are expressed from the EPI vesicle, demonstrating its functionality as a prokaryotic, transcriptionally active, membrane-bound organelle. We also show that the phage nucleus is essential, with genome replication beginning after the injected DNA is transferred from the EPI vesicle to the phage nucleus. Our results show that Chimalliviridae require two sophisticated subcellular compartments of distinct compositions and functions that facilitate successive stages of the viral life cycle.

Indexed as

BacteriophagesGenome, ViralOrganellesCell NucleusViral ProteinsVirus ReplicationViral Proteinsbacterial cell biologyEPI vesiclejumbo phagephagephage nucleusviral organelle

Identifiers

PMID40168997
PMCPMC12256282

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.