ArticleCell2025
Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic.
Article in Cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed.
- Submolecular video-imaging of the Smc5/6 complex topologically bound to DNA.Nature communications · 2026Article
- Obstacle bypassing and intrinsically asymmetric loop extrusion in the segment-capture model of Structural Maintenance of Chromosomes complexes.bioRxiv : the preprint server for biology · 2026Article
- Bacterial 3D genome architecture: organization, regulation, and synthetic biology applications.Genome biology · 2026Review
- Intratumoral delivery of probiotic Ligilactobacillus salivarius H22A013 modulates the melanoma microenvironment and enhances trametinib efficacy.BMC microbiology · 2026Article
- Involvement of the inner surface residues of bacterial SMC protein MukB in the ssDNA binding in vitro.Communications biology · 2025Article
- The SMC Hinge is a Selective Gate for Obstacle Bypass.Nature communications · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Ring-like structural maintenance of chromosome (SMC) complexes are crucial for genome organization and operate through mechanisms of DNA entrapment and loop extrusion. Here, we explore the DNA loading process of the bacterial SMC complex MukBEF. Using cryoelectron microscopy (cryo-EM), we demonstrate that ATP binding opens one of MukBEF's three potential DNA entry gates, exposing a DNA capture site that positions DNA at the open neck gate. We discover that the gp5.9 protein of bacteriophage T7 blocks this capture site by DNA mimicry, thereby preventing DNA loading and inactivating MukBEF. We propose a comprehensive and unidirectional loading mechanism in which DNA is first captured at the complex's periphery and then ingested through the DNA entry gate, powered by a single cycle of ATP hydrolysis. These findings illuminate a fundamental aspect of how ubiquitous DNA organizers are primed for genome maintenance and demonstrate how this process can be disrupted by viruses.
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Registered trials
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