ArticleThe American journal of tropical medicine and hygiene2025
Conventional DNA Extraction Followed by Real-Time PCR Had Higher Sensitivity for Detection of Mycobacterium Tuberculosis in Clinical Samples Compared to Standard Methods.
Article in The American journal of tropical medicine and hygiene, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Comparative evaluation of three commercial real-time PCR kits for direct detection ofMicrobiology spectrum · 2026Article
- Interinstitutional collaboration to promote public health research in Ecuador: a story of success from the National Institute of Public Health in Ecuador.Frontiers in public health · 2026Article
- "The end TB strategy" pathway in South America: out of track for 2025 milestones and 2035 eradication.Lancet regional health. Americas · 2025Article
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Authors and funding
13 authors.
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Abstract
The Mycobacterium tuberculosis complex (MTBC), the causative agent of tuberculosis (TB), remains a significant global health challenge, with an estimated 10.8 million cases diagnosed and 1.25 million deaths in 2023, according to the WHO. In this context, enhancing TB case detection using more sensitive diagnostic methods is essential. Here, we compared the performance of two commercial real-time polymerase chain reaction (PCR) kits with the reference standards of smear microscopy, culture, and Gene Xpert. By analyzing 402 clinical specimens, we found that conventional DNA extraction, followed by real-time PCR, using either of the two commercial kits provided the highest sensitivity for detecting MTBC. Positivity values of 48.98-60.07% and 50.34-53.24% were obtained with the commercial kits "VIASURE MTBC + non tuberculous mycobacterias (NTM) Real-Time PCR Detection Kit" (Certest, Spain) and "ANYPLEX™ MTB/NTM Real-Time Detection Kit" (Seegene, South Korea), respectively. In contrast, the reference standards yielded positivity values of 14.75% (smear microscopy), 32.65% (culture), and 28.95% (GeneXpert assay). These alternative methods should be considered as valuable tools to strengthen TB control and prevention strategy.
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